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| Mechanism of inducing mitophagy in human hepatocellular carcinoma Hep3B cells by Bakuchiol |
| Hits 241 Download times 186 Received:November 20, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2024.05.03 |
| Key Words
hepatocellular carcinoma;bakuchiol;mitophagy;cell growth inhibition |
| Author Name | Affiliation | E-mail | | ZHAO Haonan | School of Intergrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Qiang | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WEN Zhongqing | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | KANG Ning | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | kangndd@163.com |
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| Abstract
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| [Objective] To investigate the effect of bakuchiol（BAK） on the mitophagy of human hepatocellular carcinoma Hep3B cells and its Mechanism. [Methods] MTT assay was used to detect the effect of BAK and mitophagy inhibitors CQ/Baf-A1 on the proliferation of Hep3B cells；Morphological changes of Hep3B cells after BAK treatment observed by phase contrast microscopy；Hoechst/Mitotracker/Rhodamine fluorescent probe staining combined with high connotation cell imaging system was used to analyze the effect of BAK on mitochondrial function；The expression of autophagy-related proteins LAMP1，LC3，p62，and mitophagy-related proteins PINK1，Parkin，MFN2，Tom20 at different time points of BAK were detected by Western blotting. [Results] BAK inhibited the growth of Hep3B cells in the time- and dose-dependent manners；High Content Analysis results suggest that BAK can elevate mitochondrial mass and decrease mitochondrial membrane potential；The addition of mitophagy inhibitors markedly reverse the death rate of Hep3B cells induced by BAK；The proteins LAMP1，LC3，PINK1，Parkin were significantly increased by BAK in the time-dependent manners，while the p62，MFN2，Tom20 were reduced；Addition of mitochondrial autophagy inhibitor could significantly reverse the growth inhibition of Hep3B cells induced by BAK. [Conclusion] Bakuchiol induces inhibition of cell growth in Hep3B cells by activating mitophagy. |
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