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| Simultaneous determination of hesperidin，baicalin and glycyrrhizic acid in Qingjin Yiqi Granules by UPLC method |
| Hits 174 Download times 100 Received:February 17, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.06.08 |
| Key Words
Qingjin Yiqi Granules;hesperidin;baicalin;glycyrrhizic acid;UPLC;content determination |
| Author Name | Affiliation | E-mail | | ZHANG Zhixian | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shenzhen 518110, China | | | ZHANG Biqian | Tianjin Modern Innovative TCM Technology Co., Ltd., Tianjin 300384, China | | | WANG Yong | China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shenzhen 518110, China | Wangyong@999.com.cn |
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| Abstract
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| [Objective] To establish an ultra-performance liquid chromatography（UPLC） method for simultaneous determination of hesperidin，baicalin and glycyrrhizic acid in Qingjin Yiqi Granules. [Methods] The chromatography was performed on Waters CORTECS UPLC T3 column （2.1 mm×100 mm，1.6 μm） and the mobile phase was 0.1% phosphoric acid aqueous solution （A）-acetonitrile （B），which was processed as follows：11%B at 0~13 min，11%~ 15%B at 13~18 min，15%B at 18~35 min，15%~26% B at 35~45 min，26%~40%B at 45~55 min，40%B at 55~60 min. The flow rate was 0.3 mL/min，the column temperature was set at 30 ℃，the injection volume was 2μL，and the detection wavelength was 237 nm and 280 nm. [Results] The linearity of the three components in the set concentration range was good（r2≥0.999 8），the relative standard deviation（RSD） of the precision and repeatability were less than 2%，the average recoveries were 90%~99% with RSD were less than 5%，and the stability was good at room temperature for 24 h. [Conclusion] The method was stable，accurate and reproducible，which could provide a certain reference for the quality evaluation of Qingjin Yiqi Granules. |
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