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| Study on the in vitro mechanism of Physochlaina infundibularis Kuang in intervening pulmonary fibrosis |
| Hits 173 Download times 132 Received:January 28, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.06.09 |
| Key Words
Physochlaina infundibularis Kuang;pulmonary fibrosis;TGF-β1/Smad3 |
| Author Name | Affiliation | E-mail | | SHANG Dandan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHOU Hong | The Sixth Chinese Medicine Factory of Tianjin Darentang Group Co., Tianjin 300401, China | | | HUANG Manru | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIANG Yongping | The Sixth Chinese Medicine Factory of Tianjin Darentang Group Co., Tianjin 300401, China | | | LIU Caiyan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Rui | Phytochemical Research Department, Research Institute of Traditional Chinese Medicine, Tianjin 300457, China | | | ZHANG Siqi | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Haiyang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Tao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangtao@tjutcm.edu.cn |
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| Abstract
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| [Objective] To study the effects of Physochlaina infundibularis Kuang on Recombinant Mouse TGF-β1 （TGF-β1） as well as cigarette smoke extract（CSE） on mouse embryonic fibroblasts（NIH-3T3） and human bronchial epithelial cells（BEAS-2B） and related mechanisms. [Methods] MTT method was used to screen the safe concentrations of Physochlaina infundibularis Kuang alkaloids，non-alkaloids，and total extracts on NIH-3T3 cells；NIH-3T3 cells were induced by TGF-β1 for 24 h，stained with Sirius Scarlet，and the relative collagen deposition rate was calculated；MTT method was used to screen the safe concentrations of 7 Physochlaina infundibularis Kuang alkaloidal monomers on BEAS-2B cells，respectively；BEAS-2B cells were treated with CSE After 24，βgalactosidase staining was performed；Western blot method was used to detect the expression levels of TGF-β1， Smad3 and NLRP3 proteins. [Results] Physochlaina infundibularis Kuang alkaloids，non-alkaloids，and total extracts significantly inhibited TGF-β1-induced collagen deposition in NIH-3T3 cells（all P<0.05）；scopolamine and dehydro-atropine significantly inhibited CSE-induced senescence in BEAS-2B cells （all P<0.05），and were able to significantly inhibit the expression of TGF-β1，Smad3，and NLRP3 proteins （all P <0.05）. [Conclusion] Physochlaina infundibularis Kuang may regulate the progression of pulmonary fibrosis in vitro by inhibiting the TGFβ1/Smad3 pathway. |
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