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| Experimental study of Fuzheng Gubiao Decoction on immune function of immunocompromised mice |
| Hits 318 Download times 136 Received:March 06, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.06.10 |
| Key Words
Fuzheng Gubiao Decoction;immunodeficiency;cyclophosphamide;immunoregulation |
| Author Name | Affiliation | E-mail | | ZHANG Shaohui | Hunan University of Chinese Medicine, Provincial Key Laboratory of TCM Diagnostics, Changsha 410208, China | | | LI Siyuan | Hunan University of Chinese Medicine, Provincial Key Laboratory of TCM Diagnostics, Changsha 410208, China | | | WEN Mengxin | Hunan University of Chinese Medicine, Provincial Key Laboratory of TCM Diagnostics, Changsha 410208, China | | | LI Liang | Hunan University of Chinese Medicine, Provincial Key Laboratory of TCM Diagnostics, Changsha 410208, China | superliliang@126.com |
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| Abstract
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| [Objective] To study the immunoregulatory effect of Fuzheng Gubiao Decoction on immunosuppressive mice induced by cyclophosphamide. [Methods] 48 Balb/c mice were randomly divided into the control group and the model group，and the Fuzheng Gubiao Decoction group （7.15 mg/kg），and the western medicine group（levamisole hydrochloride，30 mg/kg）. In addition to the control group, the other groups were injected with cyclophosphamide intraperitoneally to prepare immunosuppression model. After the successful modeling，the Fuzheng Gubiao Decoction group and the Western medicine group were given intragastric intervention for 7 days. The general state and body weight of mice were observed，and the immune organ index was detected；the serum levels of TNF-α，IL-4 and IFN-γ were determined by ELISA；the pathological changes of spleen were observed by HE staining；the distribution and proportion of splenic lymphocyte subsets CD4+ T lymphocytes，CD8+ T lymphocytes and CD4+/CD8+ were detected by flow cytometry；the mRNA and protein expression levels of NF-κB p65 and IκBα in spleen tissues were detected by real-time quantitative PCR and Western Blot. [Results] Compared with the control group，the mice in the model group showed signs of listlessness and dull hair，accompanied by a significant decrease in body weight，spleen index and thymus index were significantly increased（P<0.01）；Serum TNF-α level was significantly increased（P<0.01）， The levels of IL-4 and IFN-γ were significantly decreased（P<0.01）；the red and white pulp of that spleen were not clearly demarcate，with the infiltration of red blood cells and inflammatory cell；and the number of CD4+ lymphocytes decreased significantly （P <0.01），the number of CD8+ lymphocytes increased significantly （P <0.01），and the proportion of CD4+/CD8+ decreased significantly（P<0.01）；the expressions of NF-κB p65 mRNA and protein in spleen were significantly decreased（P<0.01），and the expressions of IκBα mRNA and protein were significantly increased（P<0.01）.Compared with the model group，the general state of Fuzheng Gubiao Decoction group was improved，body mass was increased，spleen index and thymus index were significantly increased（P<0.01），serum TNF-α level was significantly decreased（P<0.01），IL-4 and IFN-γ levels were significantly increased（P<0.01）；and the red and white pulp lines in spleen tissue were gradually clear；The number of CD4+ lymphocytes increased，the number of CD8+ lymphocytes decreased，and the proportion of CD4+/CD8+ increased；the mRNA and protein expressions of NF-κB p65 in spleen were significantly increased（P<0.05），and the mRNA and protein expressions of IκBα were significantly decreased（P<0.05）. [Conclusion] Fuzheng Gubiao Decoction can regulate the immune state of immunocompromised mice and enhance the immune function of the body. |
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