|
| Effects of bear bile powder on bile acids and intestinal flora in type 2 diabetic mice |
| Hits 156 Download times 132 Received:March 22, 2024 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2024.07.06 |
| Key Words
bear bile powder;type 2 diabetes;bile acid;intestinal flora |
| Author Name | Affiliation | E-mail | | GUO Mengxiao | Shanghai lnnovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200135, China | | | GE Dingzuo | Shanghai lnnovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200135, China | | | WANG Ruirui | Shanghai lnnovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200135, China | | | LIU Baocheng | Shanghai lnnovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200135, China | | | ZHANG Lei | Shanghai lnnovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200135, China | zhanglei37@sina.com |
|
| Abstract
|
| [Objective] To investigate the effects of bear bile powder（BBP） on bile acid content and intestinal flora in mice with type 2 diabetes（T2D）. [Methods] In order to create T2D model mice，high-fat diets combined with an injection of streptozotocin（STZ） were used. The mice were then divided into five groups：normal control，high-fat diet，model，metformin，and BBP. The drug was given once daily for 8 weeks. Following gavage，mice were assessed for glucose metabolism level，lipid metabolism，liver function enzymes，serum fasting insulin，and lipopolysaccharide binding protein；the homeostasis model assessment-insulin resistance（HOMA-IR） index was computed；bile acid content in liver tissue was determined using UPLC-MS/MS；structural changes in mouse feces were detected using 16S rRNA gene amplicon sequencing；and the relationship between bile acid and intestinal flora was examined using Spearman correlation analysis. [Results] Prophylactic BBP administration resulted in significant reductions in the levels of fasting blood glucose（FBG），oral glucose tolerance test area under the curve（OGTT AUC），fasting insulin（FINS），HOMA-IR，total cholesterol（TC），and low-density lipoprotein cholesterol（LDL-C） when compared to the model group. Additionally，the liver’s content of dehydrohydrocholic acid，23-deoxycholic acid，porcine cholic acid，and taurocholic acid-3-sulfate was significantly increased（P＜0.05）. The relative abundances of Lactococcus，Akkermansia，and Lactobacillus were all raised by bear bile powder，whereas the richness indexes，diversity indexes，and serum LBP were considerably lowered. Spearman Correlation analysis revealed that Lactobacillus was shown to be negatively correlated with OGTT AUC，FINS，and HOMA-IR in addition to being favorably correlated with 23-demethodeoxycholic acid. Akkermansia has a negative correlation with HOMA-IR. [Conclusion] In T2D mice，BBP enhanced the metabolism of fats and carbohydrates，altered the intestinal flora，and controlled bile acid levels. |
|
|
|
|
|
