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| Exploring the mechanism of action of Danzhi Jiangtang Capsule in diabetic nephropathy based on network pharmacology and cellular experiments |
| Hits 124 Download times 66 Received:February 22, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.08.05 |
| Key Words
Danzhi Jiangtang Capsule;diabetic kidney disease;Network Pharmacology;renal tubular epithelial cell;PI3K/AKT signaling pathway |
| Author Name | Affiliation | E-mail | | RUAN Noubing | The First Clinical College, Anhui University of Traditional Chinese Medicine, Hefei 230012, China | | | XU Qi | Graduate School, Anhui University of Traditional Chinese Medicine, Hefei 230012, China | | | JING Jiawen | The First Clinical College, Anhui University of Traditional Chinese Medicine, Hefei 230012, China | | | LI Jinju | The First Clinical College, Anhui University of Traditional Chinese Medicine, Hefei 230012, China | | | LI Yufan | The First Clinical College, Anhui University of Traditional Chinese Medicine, Hefei 230012, China | | | FANG Zhaohui | The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Traditional Chinese Medicine, Hefei 230012, China
Fang Zhaohui, Anhui Famous Traditional Chinese Medicine Workshop, Hefei 230031, China | fangzhaohui9097@163.com |
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| Abstract
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| [Objective] To explore the mechanism of action of Danzhi Jiangtang Capsule in intervening diabetic kidney disease(DKD) using network pharmacology combined with cellular experiments. [Methods] The active ingredients of Danzhi Jiangtang Capsule were collected through TCMSP and HERB databases,and the targets corresponding to the active ingredients of the drugs were screened and collected using PubChem database,SwissADME and SwissTargetPrediction databases. The targets of DKD were collected through GeneCards database,OMIM database,TTD database,and DrugBank database. Cytoscape software was used to construct the network map of traditional Chinese medicine(TCM)-components-targets,STRING online software was used to construct the PPI network,and DAVID database was used to analyze the GO function and KEGG pathway enrichment of the intersecting genes. Cellular experimental validation based on network pharmacology results. [Results] The screening obtained 66 kinds of active ingredients in Danzhi Jiangtang Capsule,involving 368 targets,and 54 common targets with DKD. PPI analysis showed that Danzhi Jiangtang Capsule mainly acted on the targets of interleukin-6(IL-6),Tumor Protein 53(TP53),interleukin-1β(IL-1β),protein kinase B 1(AKT1),matrix metalloproteinase(MMP),C-C motif chemokine ligand 2(CCL2),and tumor necrosis factor(TNF),and there were 365 kinds of biological processes related to common targets,63 kinds of molecular functions,and 35 kinds of cellular components,which acted on 119 signaling pathways to play a therapeutic effect for DKD,of which phosphoinositide 3-kinase(PI3K)/AKT was the key signaling pathway. In vitro cellular experiments demonstrated that Danzhi Jiangtang Capsule-containing serum decreased the expression of proteins related to the PI3K/AKT signaling pathway. [Conclusion] In this study,we used network pharmacology to predict the core targets and pathways of Danzhi Jiangtang Capsule and verified them through in vitro experiments,indicating that Danzhi Jiangtang Capsule can exert therapeutic effects on DKD through multiple pathways-multi-targets-multi-pathways. |
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