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| Effect of curcumin on proliferation，apoptosis，and chemotherapy resistance of gastric cancer cells by regulating the PI3K/AKT/mTOR signaling pathway |
| Hits 114 Download times 112 Received:March 01, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.08.06 |
| Key Words
curcumin;gastric cancer;phosphatidylinositol 3 kinase/protein kinase B/mammalian target of rapamycin signaling pathway;apoptosis;proliferation;chemotherapy resistance |
| Author Name | Affiliation | | ZHONG Yun-feng | Department of Anorectal, Wuhan Third Hospital, Wuhan, Hubei 430070, China | | XIAO Yujie | Department of Anorectal, Wuhan Third Hospital, Wuhan, Hubei 430070, China | | XIA Gan | Department of Anorectal, Wuhan Third Hospital, Wuhan, Hubei 430070, China | | YUAN Simin | Department of Anorectal, Wuhan Third Hospital, Wuhan, Hubei 430070, China |
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| Abstract
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| [Objective] To investigate the effect of curcumin(Cur) on the proliferation,apoptosis,and chemotherapy resistance of gastric cancer cells by regulating the phosphatidylinositol 3 kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR) signaling pathway. [Methods] MGC-803 cells were separated into control group,Cur low-dose group,Cur medium dose group,Cur high-dose group,and Cur high-dose+740Y-P group for the detection of MGC-803 cell proliferation and apoptosis behavior. MGC-803/DDP cells were separated into blank group,Cur group,cisplatin(DDP) group,Cur+DDP group,and Cur+DDP+740Y-P group for the detection of chemotherapy resistance in MGC-803/DDP cells. Cloning experiments and CCK-8 were applied to detect MGC-803 or MGC-803/DDP cell proliferation;flow cytometry was applied to detect apoptosis of MGC-803 or MGC-803/DDP cells;Western blot was applied to detect the expression of anti-proliferating cell nuclear antigen(PCNA),Bcl2 associated X protein(Bax),multidrug resistance associated protein 1(MRP1),p-PI3K,p-AKT,and p-mTOR proteins in cells. [Results] Compared with the control group,the colony formation rate,proliferative ability,and expression of PCNA,p-PI3K,p-AKT,and p-mTOR proteins in MGC-803 cells reduced in the Cur low-dose group,Cur medium dose group,and Cur high-dose group,the apoptosis rate and the expression of Bax protein increased,in a dose-dependent manner(P<0.05),the results indicated that Cur could inhibit PI3K/AKT/mTOR pathway and MGC-803 cell proliferation,and induce cell apoptosis;compared with the Cur high-dose group,the colony formation rate,proliferative ability,and expression of PCNA,p-PI3K,p-AKT,and p-mTOR proteins in MGC-803 cells increased in the Cur high-dose+740Y-P group,the apoptosis rate and expression of Bax protein decreased(P<0.05),these results indicated that Cur could inhibit the proliferation of MGC-803 cells and induce apoptosis by inhibiting PI3K/AKT/mTOR pathway. Compared with the blank group,the colony formation rate,proliferative ability,and expression of PCNA,p-PI3K,p-AKT,and p-mTOR proteins in MGC-803 cells in the Cur and DDP groups reduced,the apoptosis rate increased(P<0.05),the results showed that MGC-803/DDP cells had higher drug resistance and PI3K/AKT/mTOR pathway activation than MGC-803 cells;compared with the Cur and DDP groups,the colony formation rate,proliferative ability,and expression of PCNA,p-PI3K,p-AKT,and p-mTOR proteins in MGC-803 cells in the Cur+DDP group decreased,the apoptosis rate increased(P<0.05),the results indicated that Cur could inhibit PI3K/AKT/mTOR pathway and reduce drug resistance of MGC-803/DDP cells;compared with the Cur+DDP group,the colony formation rate,proliferative ability,and expression of PCNA,p-PI3K,p-AKT,and p-mTOR proteins in MGC-803 cells in the Cur+DDP+740Y-P group increased,the apoptosis rate decreased(P<0.05),these results indicated that Cur might decrease the drug resistance of MGC-803/DDP cells by inhibiting PI3K/AKT/mTOR pathway. [Conclusion] The mechanism of Cur inhibiting MGC-803 cell proliferation,promoting cell apoptosis and reducing DDP resistance may be related to inhibition of PI3K/AKT/mTOR pathway. |
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