|
| Protective effect of isoliquiritigenin on autoimmune thyroiditis in mice based on NLRP3/Caspase-1 signaling pathway |
| Hits 88 Download times 69 Received:March 03, 2024 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2024.08.07 |
| Key Words
isoliquiritigenin;autoimmune thyroiditis;tripterygium wilfordii polyglycoside tablet;mouse |
| Author Name | Affiliation | | YANG Liping | Department of Thyroid and Breast Surgery, Longhua District People's Hospital of Shenzhen City, Shenzhen 518109, China |
|
| Abstract
|
| [Objective] To observe the protective effect of isoliquiritigenin on autoimmune thyroiditis(AIT) in mice based on nucleotide-binding oligomerization domain,leucine-rich repeat and pyrin domain-containing 3(NLRP3)/Caspase-1 signaling pathway. [Methods] Sixty Specific Pathogen Free(SPF) C57BL/6J mice were randomly divided into blank control group,model group,tripterygium wilfordii polyglycoside tablet group and isoliquiritigenin high and low dose group,with 10 mice in each group. The mouse model of AIT was established by subcutaneous injection of high iodine water and pig thyroglobulin mixture. On the day of modeling,the mice in the administration group were given oral administration for 2 weeks. After the experiment,the serum levels of autoimmune antibodies including antithyroid peroxidase autoantibody(TPOAb) and anti-thyroglobulin antibodies(TGAb) and inflammatory factors including interleukin-8(IL-8),interleukin-6(IL-6) and Interleukin-23(IL-23) were detected. HE staining was used to observe the pathological changes of thyroid tissue in each group of mice. Western blot was used to detect the expression levels of NLRP3 and Caspase-1 protein in thyroid tissue of mice in each group. [Results] Compared with the blank control mice,the levels of serum autoimmune-related antibodies(TPOAb and TGAb) and inflammatory factors(IL-8,IL-6 and IL-23) in the model group were significantly higher(P<0.01). In addition,inflammatory cells infiltrated obviously in the thyroid tissue of the model group,and the expression levels of NLRP3 and Caspase-1 protein in the thyroid tissue were significantly increased(P<0.01),suggesting that the mouse model of AIT was successfully established. Compared with the model group,the levels of serum autoimmune-related antibodies(TPOAb and TGAb) and inflammatory factors(IL-8,IL-6 and IL-23) were significantly decreased in all groups(P<0.05),the infiltration of inflammatory cells in thyroid tissue was significantly reduced,and the expression levels of NLRP3 and Caspase-1 protein in thyroid tissue were significantly decreased(P<0.05). In the isoliquiritigenin group,AIT was improved in a concentration-dependent manner. [Conclusion] Isoliquiritigenin may improve the symptoms of AIT mice by regulating NLRP3/Caspase-1 signaling pathway and inhibiting inflammation. |
|
|
|
|
|
