|
| Exploring the effect of licorice aconite on reducing cardiotoxicity based on UPLC-Q-TOF-MS and network pharmacology |
| Hits 203 Download times 58 Received:May 10, 2024 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2024.10.04 |
| Key Words
licorice;aconite;cardiotoxicity;network pharmacology |
| Author Name | Affiliation | E-mail | | CAI Minghui | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | WANG Yuming | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangyuming@tjutcm.edu.cn | | LIU Jing | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
|
| Abstract
|
| [Objective] To use ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry（UPLC-Q-TOF-MS） technology to determine the chemical components of licorice and aconite single decoction samples and combined decoction samples，and to elucidate the mechanism of licorice compatibility in reducing aconite cardiotoxicity based on network pharmacology. [Methods] The chemical compositions of single decoction and combined decoction of licorice radix and aconite radix were analysed by liquid chromatography-mass spectrometry technology to investigate the changes in chemical composition before and after the combination. Based on the results of the compositional analyses，component targets as well as cardiotoxicity targets were searched through databases such as TCMSP，David and DisGeNet. The Wayne diagram was produced，and the shared targets were entered into the String database for PPI network construction，and the network was visualised by Cytoscape software，and finally GO enrichment analysis and KEGG enrichment analysis were performed. [Results] Compared with the self-constructed database，21 compounds were identified from the single decoction of licorice root， 65 compounds from the single decoction of aconite root，and 71 compounds from the combined decoction of licorice root and aconite root. Based on liquid chromatography-mass technology，network pharmacology was applied to construct traditional Chinese medicine-active ingredient-target-disease networks，respectively. The analysis showed that there were 436 intersecting targets of licorice radix and aconite radix and cardiotoxicity disease，including AKT1，GAPDH and so on. KEGG results showed that the compatibility and detoxification of licorice radix may reduce the cardiotoxicity of aconite radix through Mitogen-Activated Protein Kinase（MAPK） signaling pathway. [Conclusion] The combination of licorice and aconite can change the toxic components of aconite，and act on AKT1，GAPDH and other targets to reduce the toxicity through MAPK，Phosphatidylinositol 3-Kinase-Protein Kinase B （PI3K-AKT） and other signaling pathways. |
|
