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| The improvement effect of low-dose Shenmai Recipe on heart failure rats by regulating HIF-1/VEGF signaling pathway |
| Hits 27 Download times 12 Received:May 10, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.10.06 |
| Key Words
Shenmai Recipe;heart failure;HIF-1/VEGF signaling pathway;angiogenesis |
| Author Name | Affiliation | E-mail | | WANG Zhe | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GUO Liuli | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Yiman | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHUANG Pengwei | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | lx19980619@163.com | | REN Ming | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin University of Traditional Chinese Medicine Baokang Hospital, Tianjin 300193, China | renming2008@126.com |
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| Abstract
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| [Objective] To explore the effect of Shenmai Recipe on angiogenesis in rats with heart failure and its effect on HIF-1/VEGF signaling pathway. [Methods] Total 55 SPF male SD rats were selected to construct HF model by intraperitoneal injection of doxorubicin hydrochloride. They were randomly divided into normal group， model group，Shenmai high-dose group and Shenmai low-dose group. There were 10 rats in the normal group and 15 rats in the other groups for 8 weeks. At the end of the experiment，cardiac function indexes were observed，and hematoxylin-eosin staining （HE），Masson pathological staining and immunohistochemical CD31 staining were performed；the levels of N-terminal pro-brain natriuretic peptide（NT-proBNP） and endothelin（ET-1） in serum were detected by ELISA kit. The level of nitric oxide（NO） in serum was detected by nitrate reductase method. The mRNA levels of interleukin-6（IL-6），interleukin-1β（IL-1β），tumor necrosis factor-α（TNF-α），vascular cell adhesion molecule-1（VCAM-1），hypoxia-inducible factor-1α（HIF-1α） and vascular endothelial growth factor A（VEGFA） were detected by RT-qPCR. Western Blot was used to detect the expression of HIF-1α and VEGFA protein. [Results] The results of cardiac function index，NT-proBNP，ET-1，NO，HE pathological staining and CD31 staining showed that the treatment group was significantly improved compared with the model group，and the overall improvement of the low-dose group was more obvious. The results of RT-qPCR showed that the expression levels of IL-6，IL-1β，TNF-α and VCAM-1 in the administration group were significantly lower than those in the model group （P<0.05 or P<0.01），and the expression levels of HIF-1α and VEGFA were significantly increased（P<0.05）. Western Blot results showed that the expression of HIF-1α and VEGFA protein in the administration group was significantly improved compared with the model group（P<0.05），and the low-dose group was significantly up-regulated compared with the model group（P<0.05）. [Conclusion] Shenmai Recipe may reduce inflammation，promote angiogenesis and improve cardiac function in rats with heart failure by regulating HIF-1/VEGF signaling pathway. |
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