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| Research on the mechanism of sedative effect of Zaoren Anshen Granules on the sleep of rats based on the analysis of cerebrospinal fluid flushing patterns |
| Hits 29 Download times 13 Received:July 07, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.10.07 |
| Key Words
Zaoren Anshen Granule;wireless electroencephalography technology;ultra-high field magnetic resonance imaging;cerebrospinal fluid;sleep;aquaporin 1;Na+-K+-Cl- cotransporter 1 |
| Author Name | Affiliation | E-mail | | LIU Hanzhi | Beijing University of Chinese Medicine, Beijing 102400, China | | | WANG Hao | Beijing University of Chinese Medicine, Beijing 102400, China | | | PAN Yanshu | Beijing University of Chinese Medicine, Beijing 102400, China | 13911209998@163.com | | ZHOU Manyuan | Beijing University of Chinese Medicine, Beijing 102400, China | | | FANG Qi | Beijing University of Chinese Medicine, Beijing 102400, China | | | SUN Jingyan | Beijing University of Chinese Medicine, Beijing 102400, China | |
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| Abstract
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| [Objective] To investigate the mechanism of Zaoren Anshen Granules in improving sleep in normal rats based on the cerebrospinal fluid secretion and flushing effect. [Methods] Forty-four male SD rats were selected and randomly divided into the normal group and the Zaoren Anshen Granule group，with 22 rats in each group. The rats in the Zaoren Anshen Granules group were given aqueous solution of Zaoren Anshen Granules for 8 consecutive days，and the normal group was controlled with an equal amount of purified water. EEG and EMG characteristics，the length of wakefulness，non-rapid eye movement（NREM） and rapid eye movement（REM） were analyzed from the 6th to the 8th day of the administration of Zaoren Anshen Granules. After 8 days of the administration of Zaoren Anshen Granules，the average flow rate of the cerebrospinal fluid in the middle portion of the midbrain ducts of the rats was detected by ultra-high-field magnetic resonance imaging equipment，and the average flow rate of the cerebrospinal fluid in the middle portion of the middle cerebral ducts of the rats was measured by ELISA. The average flow rate of cerebrospinal fluid in the middle cerebral conduit of rats was measured by ultra-high-field magnetic resonance imaging equipment，ELISA，and Western Blot for aquaporin 1（AQP1） and Na+-K+-Cl- cotransporter 1（NKCC1）. AQP1 and NKCC1 protein expression in the prefrontal cortex of the brain was detected by Western Blot. [Results] Compared with the normal group，the awakening period time was shortened（P<0.01），the sleep time was increased（P<0.01）， and both NREM period and REM period were increased（P<0.01） in the Zaoren Anshen Granule group，and the awakening time decreased day by day（P<0.01），and the sleep time was increased day by day（P<0.01） between days 6，7 and 8 in the Zaoren Anshen Granule group，and both NREM period and REM period were increased （P<0.01）， the 24 h sleep structure of normal rats was affected（P<0.01），the average flow rate of cerebrospinal fluid at the middle part of the midbrain aqueduct was significantly accelerated（P<0.05），the content of AQP1 and NKCC1 in the prefrontal cortex of the brain was significantly increased（P<0.05），and the expression of AQP1 and NKCC1 proteins in the prefrontal cortex of the brain was significantly elevated（P<0.05）. [Conclusion] Zaoren Anshen Granules significantly shortened the awakening period and lengthened the sleep period，especially the NREM and REM periods，in normal rats，and the mechanism may be related to the increase in the protein expression of cerebrospinal fluid secretion proteins，AQP1 and NKCC1，thus promoting the cerebrospinal fluid flushing. |
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