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| Experimental study on the intervention of Yiqi Huoxue Prescription on water retention in chronic heart failure based on AVP-V2R-AQP2 pathway |
| Hits 28 Download times 15 Received:May 21, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.10.08 |
| Key Words
Yiqi Huoxue Prescription;chronic heart failure;aquaporin 2;water and sodium retention |
| Author Name | Affiliation | E-mail | | WANG Mingyang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | WANG Qingquan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHANG Junping | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | tjzhtcm@163.com |
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| Abstract
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| [Objective] To observe the effects of Yiqi Huoxue Prescription on the structure of heart and kidney in rats with Chronic heart failure（CHF），and to study the mechanism from the perspective of water reabsorption. [Methods] Sprague-Dawley（SD） rats were anesthetized and the CHF model was established by ligation of the anterior descending branch of the left coronary artery，4 weeks later，the rats were randomly divided into sham operation group，model group，captopril group and Yiqi Huoxue Prescription low-dose，medium-dose and high-dose groups. Sham operation group and model group were given the same amount of distilled water by instillation. In the administration group，captopril tablets were given 13.5 mg/（kg·d），Yiqi Huoxue Prescription 2.5，5，10 g/（kg·d）， and continued gavage for 8 weeks，respectively. After the intervention，the heart structure and function of rats were analyzed by color Doppler ultrasound；serum was collected to detect the expression level of N-terminal pro brain natriuretic peptide（NT-proBNP）；Hematoxylin-eosin（HE） staining was used to observe the level of myocardial fibrosis and the morphology of renal epithelial cells. The rat renal tubular epithelial cells were analyzed after staining. The distribution and expression levels of aquaporin 2（AQP2） and pS256-AQP2 in rat kidney were analyzed by immunohistochemistry；serum argininevasopressin AVP levels were detected by Enzym-Linked ImmunoSorbent Assay（ELISA）；the expression levels of renal AQP2，pS256-AQP2 and V2 vasopressin receptor（V2R） were detected by Western Blot. [Results] Comparedwith sham operation group，ejection fraction（EF） in model group was significantly decreased（P<0.01），and serum NT-proBNP level was increased（P<0.01）. The myocardial morphology of the model group was irregular，with obvious inflammatory infiltration and renal tubule stenosis，the positive area of AQP2 and pS256-AQP2 in the model group was higher than that in the sham operation group，and the expressions of AQP2， AVP and V2R were significantly increased（P<0.01）. EF in Yiqi Huoxue groups was higher than that in model group （P<0.01），and serum NT-proBNP level was lower than that in model group（P<0.01）；the myocardial tissue kept regular， and the corresponding necrosis and apoptosis were not obvious；the positive expression area of AQP2 and pS256- AQP2 in captopril group，Yiqi Huoxue medium dose group and Yiqi Huoxue high dose group decreased（P<0.01 or P<0.05），and the protein expression levels of AQP2，pS256-AQP2，AVP and V2R in each administration group could be reduced to different degrees. [Conclusion] Yiqi Huoxue Prescription can regulate the renal reabsorption of water，improve the cardiac structure and function，and delay the progression of CHF by improving the renal regulatory mechanism. The mechanism may be related by regulating the expression of related factors in the AVP- V2R-AQP2 signaling pathway. |
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