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| The application of amplicon sequencing technology in molecular identification of traditional Chinese medicine |
| Hits 27 Download times 11 Received:May 12, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.10.11 |
| Key Words
amplicon sequencing;traditional Chinese medicine identification;molecular identification of traditional Chinese medicine |
| Author Name | Affiliation | E-mail | | ZHANG Yue | State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | ZHANG Tingting | State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | XING Zhimei | State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | XU Liuwei | State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | TIAN Xiaoxuan | State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | tian_xiaoxuan@tjutcm.edu.cn |
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| Abstract
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| Traditional Chinese mediciｎｅ （TCM） identification is a crucial step in ensuring the safety of TCM use. Molecular identification of TCM involves analyzing genetic material differences， which helps to overcome the subjectivity of traditional identification methods and serves as a complement to TCM identification. The amplicon sequencing technique amplifies specific gene fragments and combines various sequencing technologies for species identification. This review summarizes and analyzes the advantages of amplicon sequencing technology in biological identification， its applications in TCM and Chinese patent medicine， and the key technical steps in TCM identification using amplicon sequencing. It also examines the current status and future development of amplicon sequencing technology in molecular identification of TCM， providing research insights for future work in this field. |
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