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| Simultaneous quantification of 17 components in psoraleae fructus by UHPLC-MS/MS method |
| Hits 87 Download times 26 Received:May 02, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.11.03 |
| Key Words
psoraleae fructus;UHPLC-MS/MS;content determination |
| Author Name | Affiliation | E-mail | | XIA Ning | Center of Drug Safety Evaluation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Xiaoying | Center of Drug Safety Evaluation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHENG Liyuan | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Yue | Center of Drug Safety Evaluation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | | | ZHOU Kun | Center of Drug Safety Evaluation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | z.k.ken@263.net |
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| Abstract
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| [Objective] To establish a UHPLC-MS/MS method for the simultaneous determination of 17 components in 25 batches of psoraleae fructus. [Methods] The UHPLC-MS/MS method was performed on Thermofisher Scientific Hypersil GOLD（100×2.1mm 1.9μm）. The mobile phase was water（A）-acetonitrile（B）；Gradient elution；The injection volume was 3 μL. The flow rate was 0.2 mL/min and column temperature were 40 ℃. The ion source was ESI, the ion source temperature was 500 ℃. The positive and negative ions were measured simultaneously in multiple reaction detection（MRM） mode. [Results] psoralen, isopsoralen, bakuchiol, 4, 5-dehydroisopsoralidin, bavachalcone, isobavachromene, bavachinin, corylin, psoralidin, bavachin, isobavachalcone, 8-methoxypsoralen, isobavachin, neobavaisoflavone, 5-methoxypsoralen, bakuchalone, corylifol a 17 components with good linear relationship（r>0.99）, the average recoveries（n=6） were 95.66%~101.38%, RSD was 0.77%~3.04%. RSDS of precision, repeatability and stability tests were all less than 2.57%, which met the requirements of analysis. The content determination showed that the content of each component in different batches of psoralen was different. [Conclusion] The method established in this experiment is rapid, accurate and reproducible, and can be applied to the quality control of psoraleae fructus. |
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