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| The effect of cirsimaritin on rheumatoid arthritis in mice based on STING/NF-κB signaling pathway |
| Hits 105 Download times 43 Received:May 05, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.11.05 |
| Key Words
cirsimaritin;mouse;methotrexate;STING/NF-κB signaling pathway |
| Author Name | Affiliation | E-mail | | HUANG Chengshuo | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | LIN Xian | Department of Ultrasound Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | CHEN Ju | Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | ZHENG Jinchang | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | LIANG Zhen | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | GUO Weixiong | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | CHEN Jiming | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | 4856071@qq.com |
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| Abstract
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| [Objective] To evaluate the effect of thistle flavescens on collagen-induced arthritis（CIA） in mice by regulating STING/NF-κB signaling pathway. [Methods] Forty SPF grade female C57BL/6 mice were divided into blank control group（Sham）, model group（CIA）, methotrexate group（MTX, 1 mg/kg） and cirsimaritin group（JHS, 100 mg/kg）. The mice rheumatoid arthritis models were established by collagen immunoassay. After 4 weeks of drug intervention, the experiment was completed. Swelling degree of the hind PAWS, levels of TNF-α, IL-1β, IL-6, IL-10, MDA and SOD activity in serum were detected, morphological changes of the left knee joint were observed, and the expression level of STING/NF-κB pathway key proteins in the right knee joint was measured. [Results] Compared with CIA group, the swelling degree of hind PAWS in MTX group and JHS group was significantly decreased（P<0.01）, the levels of TNF-α, IL-1β, IL-6, IL-10 and MDA in serum were significantly decreased, while the activity of SOD was significantly increased（P<0.01）, the inflammatory cell infiltration of knee joint was significantly decreased, and the cartilage tissue damage was significantly alleviated. The expression levels of STING and P-NF-κB p65/NF-κB protein in knee joint were significantly decreased（P<0.01）. [Conclusion] Cirsimaritin can significantly improve rheumatoid arthritis, and its mechanism may be related to reducing inflammation and oxidative stress damage by regulating STING/NF-κB signaling pathway. |
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