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| Study on the inhibitory effect of Tangzhiqing extract on α-glucosidase in starch hydrolysis |
| Hits 481 Download times 246 Received:September 20, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2024.12.05 |
| Key Words
tarch hydrolysis;α-glycosidase inhibition;Tangzhiqing extract;hypoglycemic effect |
| Author Name | Affiliation | E-mail | | XIA Xin | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Yanfen | Department of Clinical Pharmacology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | Liyanfen2008@163.com | | HUANG Yuhong | Department of Clinical Pharmacology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | HUANG Wei | Oncology department, Chengde Traditional Chinese Medicine Hospital, Chengde 067000, China | | | CHENG Yu | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHU Mengyao | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| [Objective] To investigate the inhibitory effect of Tangzhiqing(TZQ) on α-glucosidase involved in starch hydrolysis and the pathway of TZQ intervention in starch hydrolysis,focusing on each step of starch hydrolysis. [Methods] Using carbohydrates such as starch,dextrin,maltose,and isomaltose as substrates,the inhibitory effect of TZQ on α-glucosidase amylase,dextrinase,maltase,and isomaltase involved in starch hydrolysis was investigated using in vitro enzyme inhibition kinetics. [Results] Within the concentration range of 6.88×10-5 to 5.50×10-4 mg/mL,TZQ showed no dose-dependent inhibition of amylase activity;within the concentration range of 1.10×10-4 to 6.60×10-3 mg/mL,TZQ showed no significant inhibition of dextrinase activity;within the concentration range of 5.50×10-4 mg/mL to 1.10×10-2 mg/mL,TZQ showed a good inhibitory effect on maltase activity with an IC50 value of 2.96×10-3 mg/mL;within the concentration range of 5.50×10-4 mg/mL to 1.10×10-2 mg/mL,TZQ showed a good inhibitory effect on isomaltase activity with an IC50 value of 1.77×10-3 mg/mL. [Conclusion] TZQ can interfere with starch hydrolysis by inhibiting the activity of amylase,maltase,and isomaltase,thereby reducing blood glucose levels. TZQ is a potential α-glucosidase inhibitor. |
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