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| To study the effect of Tongxinluo drug-containing serum on the proliferation of rat cardiac fibroblasts under high glucose |
| Hits 570 Download times 279 Received:August 31, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2025.01.08 |
| Key Words
rat cardiac fibroblasts;mannitol;drug-containing serum;TGF-β1/p38MAPK/CREB signalling pathway |
| Author Name | Affiliation | | ZHANG Changxi | Ningxia Hui Autonomous Region Hospital of Traditional Chinese Medicine & Institute of Traditional Chinese Medicine, Yinchuan 750021, China | | MA Qin | Ningxia Hui Autonomous Region Hospital of Traditional Chinese Medicine & Institute of Traditional Chinese Medicine, Yinchuan 750021, China | | ZHANG Anni | Jinan University, Guangzhou 510632, China | | CHEN Lijuan | Ningxia Medical University, Yinchuan 750004, China | | PING Xinchong | Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China |
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| Abstract
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| [Objective] To investigate the effect of tongxinluo（TXL） containing serum on the proliferation of rat cardiac fibroblasts under high glucose，based on the transforming growth factor β1-p38 mitogen-activated protein kinase-cyclophosphate-adenosine receptor binding protein（TGF-β1/p38MAPK/CREB） signaling pathway. [Methods] Primary rat cardiac fibroblasts were isolated and immunofluorescence staining was used to identify the cells. After successful modelling，the cells were treated with different concentrations of tongxinluo containing serum and positive drug valsartan containing serum，cell proliferation was detected by CCK-8，apoptosis was detected by flow assay，TGF-β1 protein expression was detected by immunofluorescence staining，and TGF-β1 protein expression was detected by enzyme-linked immunosorbent assay（ELISA）. Enzyme-linked immunosorbent assay（ELISA） for cysteine aspartate protease 3（Caspase-3），B lymphocyte 2（Bcl-2） and transforming growth factor β1（TGF-β1） expression levels in rat myocardial tissue and protein blotting assay（Western blot） for TGF-β1，p-p38MAPK，p-CREB protein expression levels. [Results] Immunofluorescence detection of vimentin expression could successfully identify rat cardiac fibroblasts with a positive rate of more than 90%. The 54.5 mmol/L mannitol and 25 mmol/L glucose were applied to rat cardiac fibroblasts for an optimal modelling time of 48 h. The results showed that cell proliferation and apoptosis rates in the model group were significantly increased compared with those in the control group，and the protein expression of Caspase-3，TGF-β1，Bcl-2，p-p38MAPK and p-CREB were significantly increased compared with those in the model group，Bcl-2，p-p38MAPK and p-CREB. The apoptosis rate of different concentrations of drug-containing serum group and positive drug-containing serum group was significantly lower than that of the model group，and the protein expression of caspase-3，Bcl-2，TGF-β1，p-p38MAPK and p-CREB was significantly lower，with statistically significant differences（P<0.05）. [Conclusion] The use of mannitol and glucose can lead to myocardial fibroblast damage in rats，and treatment with different doses of Tongxinluo containing serum and positive control valsartan containing serum can play a protective role in rat myocardial fibroblasts，in which the dose of Tongxinluo containing serum has the best effect on the repair of rat myocardial fibroblasts with high glucose，and may play a protective role in myocardial fibroblasts through activation of the TGF-β1-p38 MAPK-CREB signalling pathway. CREB signalling pathway. |
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