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| Mechanism of Fried glycyrrhizae decoction in improving CCl4 induced acute liver injury in mice by regulating the SIRT1/PGC-1α pathway |
| Hits 469 Download times 314 Received:December 10, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2025.03.06 |
| Key Words
Fried glycyrrhizae decoction;SIRT1/PGC-1α pathway;CCl4;acute liver injury |
| Author Name | Affiliation | E-mail | | LI Xuemei | Department of Pharmacy, Shangqiu First People's Hospital, Shangqiu 476100, China | | | LIU Yuan | Department of Pharmacy, Shangqiu First People's Hospital, Shangqiu 476100, China | | | QIAO Letian | Department of Pharmacy, Shangqiu First People's Hospital, Shangqiu 476100, China | 2485920787@qq.com | | WANG Ziliang | Department of Pharmacy, Shangqiu First People's Hospital, Shangqiu 476100, China | | | FANG Ming | Department of Pharmacology, Shangqiu Medical College, Shangqiu 476002, China | |
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| Abstract
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| [Objective] To explore the mechanism of Fried glycyrrhizae decoction in improving carbon tetrachloride(CCl4) induced acute liver injury in mice by regulating the silent information regulation 1(SIRT1)/peroxisome proliferator activated receptor-gamma coactivator-1α(PGC-1α) pathway. [Methods] Mice were randomly grouped into a control group,a CCl4 group,a low,medium,and high(Fried glycyrrhizae decoction-L,Fried glycyrrhizae decoction-M,Fried glycyrrhizae decoction-H) dose groups(2 mL/kg,4 mL/kg,8 mL/kg),a Fried glycyrrhizae decoction-H+SIRT1 inhibitor sirtinol(2 mg/kg sirtinol) group,and obeticholic acid(30 mg/kg) group,except for the control group,all other groups established mouse acute liver injury models by injecting CCl4,the general state and liver appearance of mice were observed,serum was collected,and levels of interleukin-6(IL-6),alanine aminotransferase(ALT),tumor necrosis factor-α(TNF-α),and aspartate aminotransferase(AST) were detected;liver tissue was collected,and levels of superoxide dismutase(SOD),glutaraldehyde(MDA),pathological damage,and SIRT1/PGC-1α pathway protein expression were detected. [Results] Compared with the control group,CCl4 group cells showed necrosis,inflammatory infiltration,and collagen fiber proliferation,the levels of AST,ALT,TNF-α,IL-6 in serum,and MDA in liver tissue obviously increased,the expression of SOD,SIRT1,and PGC-1α obviously reduced(P＜0.05);compared with the CCl4 group,the pathological damage in the Fried glycyrrhizae decoction-L,Fried glycyrrhizae decoction-M,Fried glycyrrhizae decoction-H groups,and obeticholic acid group was improved,the levels of AST,ALT,TNF-α,IL-6 in serum,and MDA in liver tissue obviously reduced,the expression of SOD,SIRT1,and PGC-1α obviously increased,different concentrations of Fried glycyrrhizae decoction showed statistical differences(P＜0.05),however,there was no difference between the Fried glycyrrhizae decoction-H group and the obeticholic acid group(P＞0.05);compared with the Fried glycyrrhizae decoction-H group,the Fried glycyrrhizae decoction-H+sirtinol group showed an increase in inflammatory infiltration and collagen deposition,the levels of AST,ALT,TNF-α,IL-6 in serum,and MDA in liver tissue obviously increased,the expression of SOD,SIRT1,and PGC-1α obviously reduced(P＜0.05). [Conclusion] Fried glycyrrhizae decoction regulates the SIRT1/PGC-1α pathway to improve CCl4 induced acute liver injury in mice. |
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