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| Evaluation of the pharmacological effect of Yiqi Qingfei Granules intervention on chronic fatigue syndrome model mice |
| Hits 468 Download times 195 Received:November 30, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2025.04.06 |
| Key Words
Yiqi Qingfei Granule;chronic fatigue syndrome;energy metabolism;5-HT;AMPK/PGC-1α/SIRT3 signaling pathway |
| Author Name | Affiliation | E-mail | | GAO Panwei | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XU Xingdi | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LUO Zijuan | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHANG Huilin | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Na | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LU Xingyue | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YUAN Qing | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | DING Hui | College of Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Xiaoguang | Tianjin Zhongyi Pharmaceutical Co. Ltd., Tianjin 301617, China | | | ZHANG Han | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MIAO Lin | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHAI Lijuan | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | cljuan1258@tjutcm.edu.cn |
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| Abstract
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| [Objective] To explore the anti-fatigue effect of Yiqi Qingfei Granules（YQQF） on chronic fatigue model mice. [Methods] Male balb/c mice were randomly divided into the control group，the model group，YQQF low dose group（2.4 g/kg），YQQF middle dose group（4.8 g/kg），YQQF high dose group（9.6 g/kg）. Mice in the control and model groups were given saline，and other mice were given YQQF. One hour after intragastric administration，all mice except those in the control group were subjected to swimming training for 2 hours，and the process lasted for 6 weeks. All mice were killed 24 hours after the last exhaustive swimming training. Forced swimming test and rotarod test were carried out to evaluate endurance. Contents of glycogen and adenosine triphosphate（ATP），and levels of serum urea nitrogen（BUN） and lactate（LA） were detected by biochemical assay kits. The concentrations of tryptophan and serotonin（5-HT） in brain tissue were tested by ELISA. The expression levels of Calcium/Calmodulin-dependent Protein Kinase Kinase 2（CaMKK2），Adenosine 5’-monophosphate（AMP）-activated protein kinas（AMPK），p-AMPK，peroxisome proliferator-activated receptor gamma coactivator 1α（PGC-1α） and Sirtuin 3（SIRT3） in gastrocnemius were assayed by Western blot. [Results] Compared with the model group，YQQF significantly prolonged the exhaustion swimming time and rotation time（P<0.05 or P<0.01），increased the glycogen content and ATP level（P<0.05），up-regulated p-AMPK，PGC-1α and SIRT3 protein levels（P<0.05 or P<0.01），while the levels of LA，BUN，tryptophan and 5-HT were decreased（P<0.05 or P<0.01）. However，there was no significant difference in CaMKK2 expression levels of gastrocnemius among groups（P>0.05）. [Conclusion] YQQF may exert therapeutic effects on chronic fatigue syndrome by activating the gastrocnemius AMPK/PGC-1α/SIRT3 signaling pathway and improving energy metabolism. |
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