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| Study on quality evaluation of Equiseti Hiemalis Herba by UHPLC fingerprint combined with multi-content determination |
| Hits 378 Download times 271 Received:October 25, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2025.05.04 |
| Key Words
Equiseti Hiemalis Herba;UHPLC;fingerprint;content determination |
| Author Name | Affiliation | E-mail | | WU Tiantian | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | ZHAO Peng | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LYU Zhenguo | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | OUYANG Huizi | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HE Jun | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | hejun673@163.com |
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| Abstract
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| [Objective] To establish ultra-high-performance liquid chromatography（UHPLC） fingerprint of Equiseti Hiemalis Herba and to comprehensively evaluate the quality of Equiseti Hiemalis Herba by combining chemometrics and multi-component content determination. [Methods] An ACQUITY CSH C18 chromatographic column was employed in this study，gradient elution was performed using 0.1% formic acid in water-acetonitrile as the mobile phase，and the detection wavelength was 270 nm. UHPLC fingerprints of 20 batches of Equiseti Hiemalis Herba were established，and the contents of components were determined. The fingerprints were evaluated by similarity analysis and hierarchical cluster analysis，and the differential components were found by orthogonal partial least squares discriminant analysis. [Results] There were 14 common peaks in the fingerprints of 20 batches of Equiseti Hiemalis Herba，and the similarity was greater than 0.992. Vanillic acid，kaempferol-3-O-sophoroside-7-O-glucoside，chlorogenic acid，ferulic acid，and kaempferol-3-O-sophoroside were identified by comparison with the standards. Hierarchical cluster analysis classified the 20 batches of Equiseti Hiemalis Herba into two categories，and orthogonal partial least squares discriminant analysis screened out six differential markers，and three components（kaempferol-3-O-sophoroside-7-O-glucoside，ferulic acid，vanillic acid） were identified. [Conclusion] The established analytical method is stable and reliable，and it can provide a reference for the quality evaluation of the Equiseti Hiemalis Herba. |
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