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| Research on the Process of Gastrodia elata and Armillaria mellea Complex |
| Hits 453 Download times 239 Received:November 05, 2024 |
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| DOI
10.11656/j.issn.1673-9043.2025.05.05 |
| Key Words
Gastrodia elata;Armillaria mellea;complex;antioxidation |
| Author Name | Affiliation | E-mail | | YI Yali | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Yiran | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Yuxuan | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | lsrlyx2008@126.com | | GUO Daliang | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | silenthawk@126.com |
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| Abstract
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| [Objective] To formulate a complex of Gastrodia elata and Armillaria mellea. [Methods] Liquid deep fermentation was employed to cultivate the mycelium of Armillaria mellea，which was subjected to pretreatment to devise wall material. This wall material was then combined with the Gastrodia elata mother liquid to yield the Gastrodia elata and Armillaria mellea complex. The effects of the ratio of Gastrodia elata to Armillaria mellea，complexation time，and complexation temperature on the properties of the complex were systematically examined，and the process was optimized. Additionally，free radical scavenging assays were conducted on the Gastrodia elata mother liquid，the wall material of Armillaria mellea，and the resulting complex. The release profiles of the Gastrodia elata and Armillaria mellea complex in various media at pH 1.2，pH 6.8，and pH 7.8 were also investigated. [Results] The optimal conditions for preparing the Gastrodia elata and Armillaria mellea complex were determined to be a ratio of Gastrodia elata mother liquid to Armillaria mellea wall material of 1：4，a complexation time of 12 hours，and a complexation temperature of 70 ℃. The scavenging capacity was observed in the order：Gastrodia elata and Armillaria mellea complex>Gastrodia elata mother liquid>Armillaria mellea wall material. The complex exhibited the fastest release in an acidic medium at pH 1.2. [Conclusion] The preparation process of the Gastrodia elata and Armillaria mellea complex is rational，exhibiting strong antioxidant capacity and rapid release，thus providing valuable insights for subsequent application studies. |
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