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| A comparative study on chemical constituents diffenrences between fresh and processed samples of Codonopsis Pilosula |
| Hits 390 Download times 179 Received:March 03, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.08.05 |
| Key Words
Codonopsis pilosula;local processing;UPLC-QTOF-MS;chemical constituents;quality evaluation |
| Author Name | Affiliation | E-mail | | HE Min | Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | HU Jiadong | Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China | | | ZHANG Chen | Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | DI Peng | National and Local Joint Engineering Research Center for the Breeding and Development of New Ginseng Varieties, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China | | | TANG Zhaohui | Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | CHEN Wansheng | Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China | | | QIU Shi | Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, Beijing 100700, China | davidhugh@msn.cn |
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| Abstract
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| [Objective] This study investigates the chemical composition differences between fresh Codonopsis pilosula（Dangshen） medicinal material and its decoction pieces，focusing on the tissue-specific distribution of components and the effects of post-harvest processing on their chemical profile. [Methods] Ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry（UPLC-QTOF-MS） was used to analyze the chemical components of fresh and processed Dangshen. Data were processed using MS-DIAL software and further analyzed through principal component analysis（PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA） to identify chemical markers distinguishing fresh material from decoction pieces. Additional analyses explored the chemical composition variations across different tissue sections of the decoction pieces，including the periderm，xylem，and phloem. [Results] The results revealed significant chemical composition differences between fresh Dangshen and its decoction pieces. Seventeen differential chemical markers，including alkaloids，organic acids，polyacetylenes，and phenylpropanoids，were identified，each displaying distinct variation patterns. Among these，polyacetylenes exhibited the most notable changes，with a marked enrichment in the xylem of the decoction pieces. [Conclusion] Post-harvest processing was found to have a profound impact on the polyacetylenes，the key active ingredients in Dangshen. The proportion of lobetyolin in the xylem emerged as a critical factor influencing the quality of the decoction pieces. These findings offer valuable insights into optimizing post-harvest processing，understanding quality formation，and establishing quality evaluation criteria for Dangshen medicinal material. |
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