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| Serum proteomics study of Naoxintong Capsule against myocardial infarction |
| Hits 357 Download times 133 Received:July 09, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.12.06 |
| Key Words
Naoxintong Capsule;serum proteomics;myocardial infarction;angiogenesis;apoptosis |
| Author Name | Affiliation | E-mail | | SHI Tingting | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GENG Xiao | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Mengyao | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | BAI Yuxin | Instrumental Analysis and Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Hong | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wanghongsys@126.com |
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| Abstract
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| [Objective] To investigate the mechanism of Naoxintong capsule against myocardial infarction（MI） through serum proteomics. [Methods] MI was induced by ligating the left anterior descending coronary artery in C57BL/6 mice. Naoxintong[300 mg/（kg·d）]was administered for 3 days. Differentially expressed proteins（DEPs） were screened by two-dimensional gel electrophoresis（2-DE） and Matrix assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry（MALDI-TOF-MS），validated by Western blot. Angiogenesis-related proteins were analyzed using proteomic arrays. [Results] Naoxintong reduced left ventricular infarct size and decreased cardiomyocyte apoptosis. Proteomic analysis identified 100 DEPs，including Vash1，Gpd1，Timp3，and Mfn1. Naoxintong upregulated PDGF-BB expression and activated the Akt/eNOS pathway. [Conclusion] Naoxintong exerts myocardial protective effects by synergistically regulating a triple network involving Vash1/PDGF-BB-mediated angiogenesis，TIMP3/MMP9-dominated matrix remodeling，and Mfn1-dependent mitochondrial dynamics. |
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