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| Study on the anti-triple-negative breast cancer mechanism of Brucea javanica ethanol extract |
| Hits 134 Download times 63 Received:September 13, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2026.01.05 |
| Key Words
triple-negative breast cancer;Brucea javanica;apoptosis;transcriptome sequencing |
| Author Name | Affiliation | E-mail | | DUAN Xinyu | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Yuru | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HUANG Chenyao | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Jiawen | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yanyan | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangyanyan@tjutcm.edu.cn | | WANG Yu | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangyu@tjutcm.edu.cn |
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| Abstract
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| [Objective] To explore the therapeutic effect of Brucea javanica ethanol extract（BJ-EE） on triple-negative breast cancer（TNBC） and verify its possible mechanism of action. [Methods] The ability of BJ-EE to inhibit TNBC cell proliferation after 24 h was detected by CCK-8 assay. The long-term effect of BJ-EE on TNBC cell proliferation was evaluated by colony formation assay. Morphological changes of cells after BJ-EE treatment were observed using an inverted microscope. The in vivo efficacy of BJ-EE against TNBC was evaluated based on a 4T1 tumor-bearing mouse model. Transcriptomic sequencing analysis was performed on tumor tissues from 4T1 tumor-bearing mice to explore the potential mechanism of BJ-EE in TNBC treatment. The effect of BJ-EE on TNBC cell apoptosis was detected by flow cytometry. Apoptosis-related gene and protein changes were verified by quantitative real-time polymerase chain reaction（RT-qPCR） and Western blot. [Results] CCK-8 results showed that，compared with the control group，BJ-EE significantly inhibited the viability of 4T1 and MDA-MB-231 cells（P<0.05 or P<0.01） in a dose-dependent manner. Colony formation assays showed that BJ-EE could inhibit the long-term proliferation of 4T1 and MDA-MB-231 cells（P<0.01）. Cell morphology changed significantly after BJ-EE treatment. In vivo，BJ-EE could also inhibit TNBC tumor growth. Transcriptomic sequencing suggested that the mechanism of action of BJ-EE against TNBC might be inducing apoptosis. Flow cytometry results confirmed that BJ-EE could induce apoptosis in TNBC cells. RT-qPCR detected upregulation of the apoptosis-related genes Bax and caspase-3，and downregulation of Bcl-2（P<0.05）. Western blot results showed upregulation of the pro-apoptotic protein Bax and the nuclear transcription factor-κB（NF-κB），and downregulation of the anti-apoptotic protein Bcl-2（P<0.05）. [Conclusion] Brucea javanica ethanol extract can inhibit TNBC by inducing apoptosis. |
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