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| Structural characterization and activity study of crude polysaccharide from Cuscuta chinensis |
| Hits 150 Download times 63 Received:August 06, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2026.01.06 |
| Key Words
Cuscuta chinensis crude polysaccharide;structural characterization;antioxidant;whitening |
| Author Name | Affiliation | E-mail | | HU Jing | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Yudie | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Mengjiao | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | KONG Qiaoli | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Peng | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Tianjin Key Laboratory of Chemistry and Analysis of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Han | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Nan | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | linan@tjutcm.edu.cn |
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| Abstract
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| [Objective] To prepare the crude polysaccharide from Cuscuta chinensis（CCCP） by water extraction and alcohol precipitation，characterize its structure，and investigate its antioxidant and whitening activities. [Methods] CCCP was obtained by water extraction and alcohol precipitation. The polysaccharide content was determined by the phenol-sulfuric acid method. The monosaccharide composition was analyzed using high-performance anion-exchange chromatography with pulsed amperometric detection（HPAEC-PAD，referred to as ion chromatography in the text）. The molecular weight was determined by size-exclusion chromatography coupled with multi-angle laser light scattering（SEC-MALLS）. Antioxidant capacity was evaluated in vitro through 1，1-diphenyl-2-picryl-hydrazyl radical（DPPH） and 2，2’-Azinobis-（3-ethylbenzthiazoline-6-sulphonate）（ABTS+） radical scavenging assays and a dual-luciferase reporter system measuring antioxidant response element（ARE） transcriptional activity. Whitening efficacy was assessed using a zebrafish model and an α-melanocyte-stimulating hormone（α-MSH）-induced melanogenesis model in B16 cells. [Results] The extraction yield of CCCP was 11.04%，with a polysaccharide content of 56.03%. The weight-average molecular weight was 74 565 g/mol. CCCP was primarily composed of mannose，galacturonic acid，glucose，galactose，and arabinose. CCCP enhanced the scavenging capacity against DPPH and ABTS+ radicals and increased ARE transcriptional activity. Furthermore，CCCP reduced melanin synthesis in zebrafish and inhibited melanin content and tyrosinase activity in α-MSH-stimulated B16 cells. [Conclusion] CCCP possesses notable antioxidant and whitening activities，providing a candidate natural active ingredient for potential applications in health products and cosmetics. |
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