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| Effects of modified Shenfu Decoction on the TLR4/MyD88/NF-κB signaling pathway in inhibiting cellular inflammation and oxidative stress in LPS-induced L-02 hepatocyte injury |
| Hits 154 Download times 26 Received:November 15, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2026.02.10 |
| Key Words
Modified Shenfu Decoction;sepsis;liver injury;oxidative stress |
| Author Name | Affiliation | E-mail | | LI Xue | Cangzhou Third Hospital, Cangzhou 061017, China | | | PAN Baochao | Department of Hepatology, Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061001, China | | | SUN Ying | Department of Hepatology, Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061001, China | | | WANG Jian | Department of Hepatology, Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061001, China | | | LI Yimeng | Cangzhou Third Hospital, Cangzhou 061017, China | | | WAHG Jieying | Cangzhou Third Hospital, Cangzhou 061017, China | | | YANG Guiying | Cangzhou Third Hospital, Cangzhou 061017, China | | | LIU Zhilong | Department of Hepatology, Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061001, China | 1280395467@163.com |
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| Abstract
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| [Objective] To investigate the protective effect of Modified Shenfu Decoction on lipopolysaccharide （LPS）-induced hepatocellular injury. [Methods] Twenty-seven SPF-grade SD rats were selected for acclimatization feeding，and after one week，gavage was performed to prepare drug-containing serums for the control group，Shenfu Decoction low-dose group（1 g/kg），medium-dose group（2 g/kg），and high-dose group（4 g/kg），respectively. Human normal hepatocyte L-02 cells were randomly divided into the Control Group（normal culture），LPS group（40 μg/mL LPS），and Modified Shenfu Decoction low-，medium-，and high-dose+LPS groups. After 24 hours of treatment，the expression levels of key proteins such as Toll-like receptor 4（TLR4），myeloid differentiation primary response protein 88（MyD88），nuclear factor kappa-B（NF-κB），interleukin-1β（IL-1β），and interleukin-6（IL-6）in cells were detected by Western blot. The mRNA expression levels of the corresponding genes were measured using quantitative real-time polymerase chain reaction（qPCR）。 Cell proliferation was assessed by the CCK- 8 assay， apoptosis was detected by flow cytometry，and reactive oxygen species（ROS） levels were measured using a fluorescent probe. [Results] Compared with the Control Group，LPS-induced L-02 cells showed a significant decrease in proliferative ability（P<0.01），a significant increase in protein and mRNA expression levels of inflammatory factors TLR4，MyD88，NF-κB，IL-1β and IL-6（P<0.01），a significant increase in apoptosis（P<0.01）， and significant oxidative stress with increased ROS（P<0.01）. Compared with the LPS group，the Modified Shenfu Decoction low-，medium-，and high-dose groups increased the proliferative ability of L-02 cells（P<0.01）， significantly decreased the protein and mRNA expression levels of the aforementioned inflammatory factors（P< 0.01），reduced apoptosis（P<0.01），and decreased oxidative stress and ROS levels（P<0.01）. All differences were statistically significant. [Conclusion] Modified Shenfu Decoction can ameliorate LPS-induced hepatocellular injury. Its mechanism of action may be related to the regulation of the TLR4/MyD88/NF-κB signaling pathway and the attenuation of inflammatory response and oxidative stress in L-02 cells. The in vitro results of this study provide a preliminary experimental basis for exploring the potential application of Modified Shenfu Decoction in septic liver injury. |
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