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| The effect of oridonin regulating the IL-6/STAT3 signaling pathway on proliferation，autophagy， apoptosis，and inflammatory response in microglia subjected to oxygen-glucose deprivation/reoxygenation |
| Hits 162 Download times 28 Received:December 10, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2026.03.07 |
| Key Words
oridonin;IL-6/STAT3 signaling pathway;oxygen-glucose deprivation/reoxygenation;microglia;proliferation;autophagy;apoptosis |
| Author Name | Affiliation | E-mail | | LI Lin | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | | | LI Xiangnan | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | | | YANG Songtao | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | | | LI Jianmin | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | | | ZHANG Yunhe | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | | | FU Aijun | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | | | ZHANG Zhiyong | Department of Neurosurgery, North China University of Technology Affiliated Hospital, Tangshan 063015, China | zzy1895@126.com |
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| Abstract
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| [Objective] To investigate the effect of oridonin（ORI） on proliferation，autophagy，apoptosis，and inflammatory response in microglia subjected to oxygen-glucose deprivation/reoxygenation（OGD/R） and its potential mechanism involving the interleukin-6（IL-6）/signal transducer and activator of transcription 3（STAT3） signaling pathway. [Methods] BV2 microglial cells were divided into the following groups：control，OGD/R，ORI（low，medium，and high concentration：ORI-L，ORI-M，ORI-H），and ORI combined with recombinant IL-6. After respective treatments for 24 h，cell proliferation was assessed by Cell Counting Kit-8（CCK-8） assay and 5-ethynyl-2'-deoxyuridine（EdU） staining. Autophagy was observed under a transmission electron microscope. Apoptosis was detected by flow cytometry. The levels of inflammatory factors [tumor necrosis factor-α（TNF-α），IL-18，and IL-1β] were measured using enzyme-linked immunosorbent assay（ELISA）. The protein expression levels of IL-6 and STAT3 were determined by Western blotting. [Results] Compared with the control group，the OGD/R group showed decreased cell viability and EdU-positive cell rate，along with increased number of autophagic vacuoles，apoptosis rate，levels of TNF-α，IL-18，IL-1β，and protein expression of IL-6 and STAT3（P<0.01）. Compared with the OGD/R group，the ORI-L，ORI-M，and ORI-H groups exhibited a concentration-dependent increase in cell viability and EdU-positive cell rate，and a concentration-dependent decrease in the number of autophagic vacuoles，apoptosis rate，levels of TNF-α，IL-18，IL-1β，and protein expression of IL-6 and STAT3（P<0.05）. Compared with the ORI-H group，the ORI combined with recombinant IL-6 group demonstrated decreased cell viability and EdU-positive cell rate，and increased number of autophagic vacuoles，apoptosis rate，levels of TNF-α，IL-18，IL-1β，and protein expression of IL-6 and STAT3（P<0.01）. [Conclusion] ORI can promote proliferation and inhibit autophagy，apoptosis，and inflammatory response in OGD/R-induced microglia. Its mechanism may be associated with the inhibition of the IL-6/STAT3 signaling pathway. |
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