|
| Effects of Phellodendron chinense formula granules on hyperuricemia in rats and its mechanism |
| Hits 28 Download times 5 Received:February 06, 2026 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2026.04.11 |
| Key Words
Phellodendron chinense;hyperuricemia;urate transporter;xanthine oxidase |
| Author Name | Affiliation | | MENG Xiaowei | Hebei Petro China Central Hospital, Langfang 065000, China | | FAN Ketao | Staff Hospital of Tianjin Tianzhong Heavy Machinery Group Co. Ltd., Tianjin 300400, China |
|
| Abstract
|
| [Objective] To establish a rat model of hyperuricemia and study the uric acid-lowering effect and mechanism of Phellodendron chinense （Huangbai）. [Methods] Male SD rats were randomly divided into blank control group（Control），model group（Model），Phellodendron chinense formula granule（HB） groups（18，9，4.5 g/kg），and positive drug allopurinol group（Allopurinol）. A rat hyperuricemia model was established by combined administration of yeast extract（15 g/kg），adenine（100 mg/kg），and potassium oxonate（300 mg/kg）. The uric acid-lowering effect of HB was evaluated by measuring serum uric acid（SUA） and uromodulin（UMOD） levels；xanthine oxidase（XOD） activity in serum and liver tissue；urinary uric acid（UUA） and UMOD levels；24-hour urine volume（24 h UV），24-hour uric acid excretion（24 h UUA），and fractional excretion of uric acid（FEUA）. The hepatorenal protective effects of HB were assessed by detecting serum and urine creatinine（Cr） and blood urea nitrogen（BUN） levels，kidney and liver weights and coefficients. Pathological changes in renal tissue were observed by HE staining. RT-PCR was used to detect mRNA expression of hepatic XOD and renal URAT1，GLUT9，UMOD，OAT1，OAT3，and ABCG2 to explore the mechanism. [Results] 1） Compared with the model group，the HB groups showed decreased serum UA，24 h UV，and 24 h UUA（P<0.05 or P<0.01），increased FEUA and urinary UA（P<0.05 or P<0.01），and reduced XOD activity in serum and liver tissue（P<0.05 or P<0.01）. Kidney weight and coefficient，liver weight and coefficient，and serum Cre and BUN levels were decreased in the HB groups（P<0.05 or P<0.01），and renal pathological damage was alleviated. 2） Compared with the blank group，the model group showed significantly downregulated renal expression of OAT1，OAT3，ABCG2，URAT1，and UMOD（P<0.05 or P<0.01），and significantly upregulated hepatic XOD and renal GLUT9 expression（P<0.01）. Compared with the model group，the HB groups upregulated renal OAT1，OAT3，ABCG2，URAT1，and UMOD expression（P<0.05 or P<0.01）. The HB 18 g/kg and 9 g/kg groups downregulated renal GLUT9 and hepatic XOD expression（P<0.05 or P<0.01）. [Conclusion] Phellodendron chinense formula granules have a therapeutic effect on hyperuricemia and alleviate renal pathological damage in hyperuricemic rats. The uric acid-lowering mechanism is related to the regulation of hepatic xanthine oxidase and renal urate transporters. |
|
