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| Fingerprint and multi-component quantitative analysis and difference comparison of volatile components between Ligusticum Chuanxiong Hort. and Angelica sinensis（Oliv.）Diels |
| Hits 172 Download times 49 Received:January 08, 2026 |
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| DOI
10.11656/j.issn.1673-9043.2026.05.06 |
| Key Words
Ligusticum Chuangxiong hort.;Angelica sinensis（Oliv.） Diels;Ultra Performance Convergence Chromatography;fingerprint;chemical pattern recognition;content determination |
| Author Name | Affiliation | E-mail | | WANG Xianlin | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China | | | GAO Xinying | School of Chinese Materia Medica, Tianjin University of Chinese Medicine, Tianjin 301617, China | | | BIAN Ziyan | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China | | | WANG Shuo | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China | | | ZHANG Han | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China | | | JIANG Miaomiao | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China | | | CHAI Xin | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | | | ZHANG Peng | National Key Laboratory of Chinese Medicine Modernization, Tianjin 301617, China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China
Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China | zhangpeng@tjutcm.edu.cn |
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| Abstract
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| [Objective] To establish an Ultra Performance Convergence Chromatography（UPC2）fingerprint and multi-component content determination method for the volatile components of Ligusticum chuanxiong and Angelica sinensis，and to compare the differences in volatile components between the two herbs. [Methods] Supercritical fluid chromatography（SFC）was used to extract the volatile components from L. chuanxiong and A. sinensis using CO2 as the mobile phase and methanol as a modifier. A Waters Viridis® BEH column（3.0×100 mm，130 Å，1.7 μm）was employed to establish the fingerprint and content determination methods，with CO2 as the mobile phase and 0.1% formic acid in methanol（v/v）as the modifier. Chemical pattern recognition was conducted on the common peaks of the two herbs to identify the key differential components. [Results] Eighteen common peaks were identified in 11 batches of L. chuanxiong，with a similarity greater than 0.94. Eleven common peaks were identified in 11 batches of A. sinensis，with a similarity greater than 0.96. Seven major common peaks were identified by reference standards， namely ligustilide，senkyunolide A，levistolide A，ferulic acid，senkyunolide H，coniferyl ferulate，and senkyunolide I. The contents of these seven chemical components in L. chuanxiong were higher than those in A. sinensis，among which the key differential components were ligustilide，senkyunolide A，and coniferyl ferulate. [Conclusion] The UPC2 fingerprint and multi-component quantitative methods for L. chuanxiong and A. sinensis were demonstrated to be rapid，stable，reliable，and environmentally friendly，which can serve as a reference for improving the quality control standards of these two herbs. The lower content of volatile components in A. sinensis than in L. chuanxiong may be the material basis for their differences in efficacy. |
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