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| Establishment of an HPLC-CAD fingerprint and multi-component content determination of Siegesbeckiae Herba |
| Hits 69 Download times 52 Received:February 17, 2026 |
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| DOI
10.11656/j.issn.1673-9043.2026.06.06 |
| Key Words
Siegesbeckiae Herba;high performance liquid chromatography;charged aerosol detector;fingerprint;content determination;kirenol |
| Author Name | Affiliation | E-mail | | JIA Zhaoyu | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SHI Jiaxin | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | QIN Han | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WEN Jiake | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MIAO Xiaoyu | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Jialing | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHANG Yanxu | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | tcmcyx@126.com | | FANG Shiming | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | fang_shiming@163.com |
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| Abstract
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| [Objective] To establish an integrated analytical method combining HPLC-CAD fingerprint and simultaneous multi-component quantification of Siegesbeckiae Herba，and to provide a reference for its quality evaluation. [Methods] An Eclipse Plus C18 column（4.6 mm×250 mm，5 μm） was used with a mobile phase consisting of 0.1%（v/v） formic acid in water（A） and acetonitrile（B） for gradient elution. The injection volume was 10 μL，and the column temperature was 35 ℃. The CAD atomizing chamber temperature was 35 ℃，the acquisition frequency was 10 Hz，and the filter constant was 5.0 s. HPLC-CAD fingerprints of 21 batches of Siegesbeckiae Herba from different regions were established，and the contents of eight indicator components were determined. [Results] The similarities of the 21 batches of fingerprints ranged from 0.912 to 0.978，and 17 common peaks were assigned. Among them，eight common peaks were identified as chlorogenic acid，rutin，3，4-dicaffeoylquinic acid，4，5-di-O-caffeoylquinic acid，kirenol，darutoside，hythiemoside B，and darutigenol. The eight components showed good linearity within their respective concentration ranges（r≥00.999 3）. The method exhibited good precision，repeatability，and sample stability，with relative standard deviations（RSD） all below 5.00%. The average recovery rates ranged from 100.87% to 102.99%，with RSDs≤1.71%. [Conclusion] The established HPLC-CAD fingerprint and multi-component content determination method are accurate and stable，and can effectively evaluate the quality of Siegesbeckiae Herba. |
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