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| Preliminary exploration of the mechanism by which Banxia Shumi Decoction improves insomnia based on the gut microbiota |
| Hits 77 Download times 75 Received:February 15, 2026 |
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| DOI
10.11656/j.issn.1673-9043.2026.06.09 |
| Key Words
insomnia;Banxia Shumi Decoction;gut microbiota;16S rRNA;intestinal barrier proteins;inflammatory |
| Author Name | Affiliation | E-mail | | WANG Jing | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | | | JIA Xiaoxu | Department of Pharmacy, Ordos Hospital of Traditional Chinese Medicine, Ordos 017010, China | | | ZHANG Yibo | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | | | LI Feifan | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | | | ZHU Jinqiang | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | zhujinqiang1860@163.com | | ZHENG Wenke | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China | zhengwk2020@tjutcm.edu.cn |
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| Abstract
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| [Objective] To investigate the effects of Banxia Shumi decoction on the gut microbiota in a mouse model of insomnia using 16S rRNA sequencing，and to preliminarily explore its underlying mechanisms. [Methods] 60 SPF-grade female C57BL/6JNifdc mice were randomly divided into a blank group，a model group，a positive drug group，and low-，medium-，and high-dose Banxia Shumi decoction groups. Except for the blank group，mice in other groups received intraperitoneal injections of PCPA（400 mg/kg） combined with a water platform for three days to establish the model. After seven days of treatment，an open field test was conducted. Subsequently，fecal samples were collected for 16S rRNA sequencing to analyze the richness and species composition of the gut microbiota in mice. Immunofluorescence and Western blot were used to detect the expression levels of intestinal barrier proteins. Enzyme-linked immunosorbent assay was employed to measure the contents of 5-Hydroxytryptamine（5-HT） and Gamma-Aminobutyric Acid（GABA） in the hypothalamus and Interleukin-6（IL-6） in the colon. Hematoxylin and eosin（HE） staining was used to observe pathological damage in the mouse hypothalamus. Immunohistochemistry was applied to examine the expression of Ionized calcium-binding adapter molecule-1（Iba-1） in the mouse hypothalamus. [Results] Treatment with Banxia Shumi decoction increased the total distance traveled，average speed，and rearing frequency in mice，while reducing resting time. It also enhanced the richness and diversity of the gut microbiota，up-regulated the expression of intestinal barrier proteins，increased the contents of 5-HT and GABA in the hypothalamus，reduced colonic inflammation levels，alleviated pathological damage in the hypothalamus，and inhibited microglial activation. [Conclusion] Banxia Shumi decoction can alleviate pathological damage in the hypothalamus of mice with insomnia，exerting anti-insomnia effects by protecting the intestinal barrier，inhibiting inflammatory responses in the gut and brain，and modulating the gut microbiota. |
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