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| Simultaneous determination of multi-constituents in Polygonum cuspidatum using multiple reaction monitoring coupled with Box-Behnken response surface methodology |
| Hits 221 Download times 284 Received:May 29, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.10.04 |
| Key Words
Polygonum cuspidatum;single factor optimization;Box-Behnken response surface methodology;multiple reaction monitoring;ＵHＰLC-MS/MS;content determination |
| Author Name | Affiliation | E-mail | | SUN Huihui | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MENG Xue | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WEN Jiake | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GAO Chuanyuan | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CANG Ru | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHANG Yanxu | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Modern Traditional Chinese Medicine Haihe Laboratory, Tianjin 301617, China | | | DU Kunze | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Modern Traditional Chinese Medicine Haihe Laboratory, Tianjin 301617, China | dkztcm@tjutcm.edu.cn | | HAO Jia | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | haojiatjtcm@126.com |
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| Abstract
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| [Objective] To achieve accurate quantification of seven active components in different batches of Polygonum cuspidatum by employing ultra-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) with multiple reaction monitoring(MRM) mode coupled with Box-Behnken response surface methodology. [Methods] The ultrasonic extraction conditions for Polygonum cuspidatum were optimized using single-factor experiments and Box-Behnken response surface design. A simultaneous quantification method for seven components(polydatin,epicatechin gallate,torachrysone-8-O-glucoside,resveratrol,emodin-1-O-glucoside,emodin-8-O-glucoside, and emodin) in Polygonum cuspidatum was established using UHPLC-MS/MS with MRM mode. This method was applied for quality assessment of 17 batches of Polygonum cuspidatum from different geographical origins. [Results] The optimized extraction conditions were determined as 72% methanol,a solid-to-liquid ratio of 1:150,and ultrasonic extraction for 30 min. Under these conditions,all seven chemical components in the 17 batches were accurately quantified. Methodological validation demonstrated good linearity for all compounds within their respective concentration ranges(r2 ≥ 0.995),with recovery rates ranging from 96.2% to 108.2%. The relative standard deviations(RSD) for inter-day and intra-day precision were ≤ 3.12% and ≤ 3.01%,respectively. Quantitative analysis revealed significant variations in the contents of the seven compounds among the 17 batches,with emodin,polydatin,and emodin-8-O-glucoside being the most abundant. [Conclusion] The established UHPLC-MS/MS method proves to be efficient,reliable,accurate,and stable for the simultaneous quantification of multiple components in Polygonum cuspidatum,providing a valuable reference for constructing a comprehensive quality evaluation system for this herb. |
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