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| Mechanistic study of Tangliping Capsule in the intervention of type 2 diabetes mellitus |
| Hits 210 Download times 113 Received:May 17, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.10.06 |
| Key Words
Tangliping Capsule;type 2 diabetes mellitus;network pharmacology;PI3K/Akt signaling pathway |
| Author Name | Affiliation | E-mail | | CAO Ningning | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | LIU Ying | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | XU Yajie | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | YUN Nairu | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | yunnairu987@126.com |
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| Abstract
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| [Objective] Predicting the potential mechanism of action of Tangliping Capsule in the treatment of type 2 diabetes mellitus(T2DM) using network pharmacology methods and molecular docking techniques,and to conduct the preliminary verification through in vitro experiments. [Methods] The active ingredients of Tangliping Capsule were screened using TCMSP database,and the targets of active ingredients were predicted and screened through the Swiss Target Prediction database. The targets related to T2DM were searched through Genecard,OMIM and TTD databases,and the common targets of Tangliping Capsule and T2DM were obtained by VENN online tool. Then,the common targets were imported into the STRING online database for protein interaction(PPI) network construction and analysis,in order to screen out the core targets of Tangliping Capsule for the treatment of T2DM. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed using the David database. The core target and the active ingredients were validated using molecular docking with AutoDockTools. Cell experiments were further used to verify:HepG2 cells were selected,insulin-induced insulin resistance(IR) model was used with high concentration of insulin. The expression of PI3K/Akt signaling pathway-related proteins was detected by Western blotting methods. [Results] A total of 34 active components and 649 target components of Tangliping Capsule were obtained. The 1 562 disease targets of T2DM were obtained,and 193 common targets of drugs and diseases were obtained. A total of 30 core targets are obtained by constructing protein interaction network diagram. A total of 334 results were obtained from GO enrichment and 138 results were obtained from KEGG pathway enrichment analysis. The main pathways included PI3K-Akt signaling pathway,AGE-RAGE signaling pathway in diabetic complications,EGFR tyrosine kinase inhibitor resistance,HIF-1 signaling pathway. Molecular docking results showed that the core targets(Akt1 and EGFR) and 29 active components had good affinity and binding activity. Results of the experimental testing prove that Tangliping Capsule could significantly elevate the glucose consumption in insulin resistance cell models(P<0.01),enhance the p-PI3K and p-Akt protein expression(P<0.01). [Conclusion] Tangliping Capsule may increase the glucose uptake of insulin resistance cells through PI3K/Akt signaling pathway and exert its anti-type 2 diabetes effect,providing theoretical reference and scientific basis for subsequent clinical application and research. |
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