|
| Total flavonoids from Drynariae rhizome promote growth plate chondrocyte proliferation via miR-125b-2-3p-mediated COL2A1 regulation in rats |
| Hits 414 Download times 190 Received:April 11, 2025 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2025.07.05 |
| Key Words
Drynariae rhizome total flavonoids;Ihh-PTHrP pathway;COL2A1;miR-125b-2-3p;rat;growth plate chondrocyte |
| Author Name | Affiliation | E-mail | | GENG Jie | Henan Provincial Orthopedic Hospital, Luoyang 471002, China | | | LIU Huanhuan | Henan Provincial Orthopedic Hospital, Luoyang 471002, China | lhhdoctor@126.com | | LI Yang | Henan Provincial Orthopedic Hospital, Luoyang 471002, China | | | WEI Bingyi | Henan Provincial Orthopedic Hospital, Luoyang 471002, China | | | XIE Yan | Henan Provincial Orthopedic Hospital, Luoyang 471002, China | |
|
| Abstract
|
| [Objective] To investigate how total flavonoids from Drynariae rhizome（TFD） promote rat growth plate chondrocyte proliferation through miR-125b-2-3p-mediated regulation of COL2A1 and the Ihh-PTHrP signaling pathway. [Methods] Growth plate chondrocytes from 2-week-old SD rats were isolated and cultured. After identification：optimal TFD concentration（250 mg/L） was determined by MTT assay. PTHrP-inhibiting plasmids were screened via qRT-PCR. Six experimental groups were established：blank control，TFD treatment，Ihh-PTHrP activation，PTHrP inhibition，TFD+PTHrP，TFD+PTHrP inhibitor. Cell proliferation（MTT），COL2A1 expression（ELISA），and miRNA screening（bioinformatics+qRT-PCR） were performed. miRNA-COL2A1 interactions were validated by dual-luciferase reporter assays. [Results] TFD（250 mg/L） maximally promoted proliferation（P<0.05）. Compared with the control group，the total flavonoids from TFD treatment group showed：significantly enhanced cell proliferation capacity，markedly downregulated miR-125b-2-3p expression，substantially upregulated COL2A1 gene expression. Dual-luciferase confirmed miR-125b-2-3p directly targets COL2A1，miR-125b-2-3p inhibitor mimicked TFD effects，while mimics antagonized them. [Conclusion] TFD enhances chondrocyte proliferation by downregulating miR-125b-2-3p to increase COL2A1 expression in the Ihh-PTHrP pathway，suggesting therapeutic potential for growth plate disorders. |
|
