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| Mechanism of Yinao Tongluo Formula in treating vascular dementia by regulating microglia-mediated neuroinflammation |
| Hits 362 Download times 212 Received:April 26, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.07.07 |
| Key Words
Yinao Tongluo Formula;vascular dementia;microglia;neuroinflammation;cytokine |
| Author Name | Affiliation | E-mail | | CHEN Yufei | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Xiaoli | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Qianqian | Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China | | | DIAO Huaqiong | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Xiaolu | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | | | QU Miao | The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China | ivy_miao@163.com |
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| Abstract
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| [Objective] To investigate the therapeutic mechanism of Yinao Tongluo Formula（YTF） on vascular dementia（VaD） through microglial activation-mediated neuroinflammation regulation. [Methods] VaD model was induced by bilateral common carotid artery occlusion（BCCAO）. Rats were divided into：sham-operated，model，western medicine（donepezil 1 mg/kg），and YTF low-（3 g/kg），medium-（6 g/kg），and high-dose（12 g/kg） groups（n=12/group） for 28-days treatment. Cognitive function was assessed by Morris water maze test. Hippocampal morphology was examined via hematoxylin-eosin（HE） staining. Interleukin（IL）-1β and IL-10 levels were measured by ELISA. Microglial activation was evaluated using CD68 immunofluorescence. [Results] Compared with sham group，model group showed：prolonged escape latency（P<0.05），reduced platform quadrant residence time/distance（P<0.05），hippocampal neuronal loss with pyramidal cell degeneration，increased IL-1β（P<0.05） and decreased IL-10（P<0.05）. Elevated CD68+ microglia（P<0.05）. YTF treatment significantly：improved cognitive performance（high-dose most effective，P<0.05），preserved neuronal architecture with dose-dependent effects，reduced IL-1β and increased IL-10（all doses，P<0.05），suppressed microglial activation（high-dose，P<0.05）. [Conclusion] YTF ameliorates VaD by inhibiting microglia-mediated neuroinflammation，possibly through IL-1β/IL-10 pathway modulation. |
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