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| Therapeutic effects of Ershen Zhenwu Decoction on inflammatory response and apoptotic pathways in chronic heart failure rats |
| Hits 296 Download times 198 Received:April 10, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.07.08 |
| Key Words
chronic heart failure;Ershen Zhenwu Decoction;network pharmacology;molecular mechanism |
| Author Name | Affiliation | E-mail | | LIU Yulong | Anhui University of Chinese Medicine, Hefei 230012, China | | | ZHANG Maomao | Anhui University of Chinese Medicine, Hefei 230012, China | | | WANG Xinyue | Anhui University of Chinese Medicine, Hefei 230012, China | | | GE Lan | Anhui University of Chinese Medicine, Hefei 230012, China
The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China | | | CHENG Xiaoyu | The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China | cxy478@163.com |
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| Abstract
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| [Objective] To investigate the molecular mechanisms of Ershen Zhenwu Decoction（EZD） in treating chronic heart failure（CHF） through network pharmacology and experimental validation. [Methods] Active components and targets of EZD were screened using TCMSP and UniProt databases，while CHF-related targets were identified from GeneCards and OMIM databases. Protein-protein interaction networks of intersecting targets were constructed using STRING and visualized with Cytoscape 3.8.2. GO and KEGG enrichment analyses were performed using DAVID database. Thirty-five rats were randomly divided into normal，model，low-dose EZD，high-dose EZD，and control groups. The CHF model was established by intraperitoneal injection of doxorubicin. Serum levels of NT-proBNP，IL-6， and TNF-α were measured，and myocardial histopathology was examined by HE staining. The expression of apoptosis-related proteins was detected by Western blot and qRT-PCR. [Results] The study identified 85 active components，174 drug targets，and 118 drug-disease intersecting targets. Core targets included AKT1，Bcl-2，MMP9，TNF，CASP3，and IL-6. GO analysis revealed 810 enriched terms，while KEGG analysis showed significant associations with TNF，apoptosis，PI3K-Akt，and MAPK signaling pathways. The component-target network demonstrated strong associations between catechin，paeoniflorin，tanshinoneⅡA and the intersecting targets. Compared with normal group，model group showed elevated NT-proBNP，IL-6，TNF-α，Caspase-3 and Bax（P<0.05），decreased Bcl-2（P<0.05），and increased inflammatory infiltration. EZD treatment reversed these changes（P<0.05） and improved myocardial pathology. [Conclusion] EZD exerts cardioprotective effects in CHF rats，potentially through modulating inflammatory responses and inhibiting apoptosis. |
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