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| Sanhuang Yishen Capsule protects renal function in diabetic kidney disease mice by inhibiting podocyte pyroptosis |
| Hits 392 Download times 216 Received:March 03, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.07.09 |
| Key Words
Sanhuang Yishen Capsule;pyroptosis;renal function;diabetic kidney disease |
| Author Name | Affiliation | E-mail | | CHEN Yangyang | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China | | | LI Bo | College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan 063210, China | | | LI Meng | Hebei Medical University, Shijiazhuang 050011, China | | | LYU Shuquan | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | LI Yuling | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | LIU Zhen | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | LI Jianxiu | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | WANG Yanan | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | WU Rui | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | WANG Lixin | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | HAN Qingqing | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | ZHANG Zhongyong | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China
Key Research Laboratory of Diabetic Kidney Disease Syndrome and Treatment, Cangzhou 061001, China | | | LIU Zhilong | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Hebei Province, Cangzhou 061001, China | 1280395467@qq.com |
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| Abstract
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| [Objective] To establish a diabetic kidney disease（DKD） mouse model and investigate the effects of Sanhuang Yishen Capsule（SHYS） on renal podocytes and its mechanism，while observing changes in renal function. [Methods] Thirty-two SPF-grade male C57BL/6 mice were adaptively fed for 1 week. Except for the normal group，DKD models were induced by intraperitoneal streptozotocin（STZ） injection and high-fat diet. Successfully modeled mice were divided into the model group，SHYS group，and captopril group. After 1 week of stabilization，SHYS and captopril groups received daily gavage for 8 weeks，while the normal and model groups received saline. Body weight and blood glucose were monitored. Serum creatinine（Scr），blood urea nitrogen（BUN），24-hour urinary protein（24 h-UP），triglycerides（TG），total cholesterol（TC），and low-density lipoprotein（LDL） were measured. Renal pathology was assessed via hematoxylin-eosin（HE） and periodic acid-Schiff（PAS） staining. Caspase-1 expression and pyroptosis were detected by immunofluorescence. Synaptopodin and Caspase-1 protein levels were analyzed by Western blot. [Results] Compared with the normal group，the model group showed increased body weight，blood glucose，24 h-UP，Scr，TC，and LDL（P<0.05 or P<0.01）. After treatment，SHYS and captopril groups exhibited reduced body weight，blood glucose，24 h-UP，Scr，and TC（P<0.05 or P<0.01），with no significant changes in BUN or TG（P>0.05）. HE/PAS staining revealed severe renal injury in the model group，which was alleviated by SHYS and captopril. Immunofluorescence and Western blot confirmed that SHYS downregulated Caspase-1（P<0.01） and upregulated Synaptopodin（P<0.05） compared to the model group. [Conclusion] SHYS ameliorates renal dysfunction in DKD mice by reducing proteinuria，improving lipid metabolism，and suppressing podocyte pyroptosis via Caspase-1 inhibition. |
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