|
| The effects of curdione on osteoarthritis in mice based on Wnt/β-catenin signaling pathway |
| Hits 327 Download times 172 Received:March 20, 2025 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2025.09.05 |
| Key Words
curdione;osteoarthritis;mice;Wnt/β-catenin signaling pathway |
| Author Name | Affiliation | E-mail | | HUANG Chenshuo | School of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China | | | CHEN Ju | Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | LIN Hao | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | LIANG Zhen | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | ZHENG Jinchang | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | GUO Weixiong | Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China | | | ZENG Li | School of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China | Lzeng@must.edu.mo |
|
| Abstract
|
| Objective To investigate the effects of curdione on osteoarthritis in mice by regulating Wnt/β-catenin signaling pathway. Methods Thirty SPF grade male C57BL/6 mice were randomly divided into Sham operation group(Sham), model group(OA) and curdione group(ESET, 60 mg/kg), with 10 mice in each group. The osteoarthritis model was constructed by medial meniscus instability. The drug was given by continuous gavage for 4 weeks. The morphological changes of knee joint were observed by saffrine O and HE staining, and serum inflammatory factors(TNF-α, IL-1β, IL-6, IL-10) were detected by ELISA and oxidative stress indexes(GSH-PX, SOD, MDA) were detected by chemiluminescence. The expression levels of key proteins in Wnt/β-catenin pathway were detected by immunohistochemistry and Western Blot. Results Compared with Sham group, a large number of inflammatory cells infiltrated the knee joint of mice in OA group, cartilage tissue was seriously damaged, serum levels of TNF-α, IL-1β, IL-6, IL-10 and MDA were significantly increased(P < 0.01), while the activities of SOD and GSH-PX were significantly decreased(P < 0.01). The relative expression levels of wnt3a and β-catenin in knee joint were significantly increased(P < 0.01). The above indexes can be improved obviously after curdione intervention. Conclusion Curdione may ameliorate osteoarthritis by regulating the Wnt/β-catenin signaling pathway to reduce inflammation and oxidative stress damage. |
|
|
|
|
|
