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| Effects of curcumin on proliferation, migration, invasion and apoptosis of endometrial carcinoma HEC-1-B cells through p53/AMPK signaling pathway |
| Hits 319 Download times 174 Received:April 18, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.09.06 |
| Key Words
curcumin;AMPK;compound C;endometrial cancer |
| Author Name | Affiliation | E-mail | | WEN Mengke | Uterine Corpus Tumor Ward of Gynecological Center, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830001, China | | | ZHAO Shihong | Uterine Corpus Tumor Ward of Gynecological Center, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830001, China | | | SHEN Guqun | Uterine Corpus Tumor Ward of Gynecological Center, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830001, China | 382709897@qq.com |
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| Abstract
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| Objective Exploring the biological effects of curcumin on human endometrial cancer cell line HEC-1-B by affecting the AMPK signaling pathway. Methods Firstly, HEC-1-B cells were treated with different concentrations of curcumin(0, 5, 10, 20, 40 μmol/L), and the inhibition rate of cell proliferation was detected by MTT assay. Divided HEC-1-B cells into control group(untreated) and compound C group(10 μmol/L), Curcumin group(20 μmol/L) and curcumin(20 μ mol/L)+compound C(10 μmol/L group), the drug action time was 48 hours. The proliferation of HEC-1-B cells was detected by MTT method, and the migration and invasion of HEC-1-B cells were detected by scratch assay and transwell, respectively. The apoptosis of HEC-1-B cells was detected by flow cytometry. The expression levels of caspase 3, Bcl-2, Bax mRNA in HEC-1-B cells were detected by RT-PCR, and the expression of caspase 3, Bcl-2, Bax, and AMPK signaling pathway proteins in HEC-1-B cells were detected by Western blot. Results Compared with 0 μmol/L, the proliferation inhibition rate of 5 μmol/L curcumin was not significant(P>0.05). The proliferation inhibition rates of 10 μmol/L, 20 μmol/L, and 40 μmol/L curcumin were significantly increased(P < 0.05) and showed a dose-dependent effect; Compared with the control group, the curcumin group showed a significant decrease in cell proliferation, migration, and invasion(P < 0.05), a significant increase in apoptosis(P < 0.05), an increase in caspase 3 and Bax expression(P < 0.05), and a decrease in Bcl-2 expression(P < 0.05);The expression of p-AMPK and p-p53 proteins increased(P < 0.05);In the compound C group, cell proliferation, migration, and invasion were significantly increased(P < 0.05), apoptosis was significantly reduced(P < 0.05), caspase 3 and Bax expression were reduced(P < 0.05), and Bcl-2 expression was increased(P < 0.05);The expression of p-AMPK and p-p53 proteins decreased(P < 0.05);There was no significant change in various indicators of cells in the curcumin+compound C group(P>0.05). Conclusion Curcumin affects the proliferation, migration, invasion, and apoptosis of endometrial cancer HEC-1-B cells by interfering with the p53/AMPK signaling pathway. |
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