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| Effects of Changpu Yujintang on recurrent epilepsy mice based on TLR4/NF-κB signaling pathway |
| Hits 335 Download times 150 Received:May 17, 2025 |
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| DOI
10.11656/j.issn.1673-9043.2025.09.07 |
| Key Words
Changpu Yujintang;recurrent epilepsy;neuroinflammation;TLR4/NF-κB signaling pathway |
| Author Name | Affiliation | E-mail | | HU Yanan | College of Integrated Traditional Chinese and Western Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YUE Wei | Neurology Department of Tianjin Huanhu Hospital, Tianjin 300350, China | hhyuewei2008@163.com | | LING Ling | Neurology Department of Tianjin Huanhu Hospital, Tianjin 300350, China | | | TIAN Zhen | Neurology Department of Tianjin Huanhu Hospital, Tianjin 300350, China | | | LI Wenxia | Neurology Department of Tianjin Medical University, Tianjin 300070, China | | | FAN Weijia | Tianjin Huanhu Hospital Laboratory, Tianjin 300350, China | |
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| Abstract
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| Objective To study the mechanism of Changpu Yujintang(CPYJT) in the treatment of recurrent epilepsy by improving neuroinflammation. Methods Twelve SPF C57BL/6 mice were selected as blank group, and another 60 status epilepticus(SE) mice were randomly divided into 5 groups: model group, low, middle and high dose of CPYJT group and sodium valproate group. SE mice were injected intraperitoneally(ip) Kainic acid(KA) 25 mg/kg to establish SE model. After the model was successfully established, normal saline was given to the mice. The low, middle and high doses of CPYJT [2.05、4.1、8.2 g/(kg·mL)]and sodium valproate sustained-release tablets [0.13 g/(kg·mL)]were given intragastrically for one week. Ip KA 25 mg/kg again 1 week later. The pathological changes of CA3 and CA1 regions of hippocampus in mice were observed by hematoxylin-eosin(HE) staining and Nissl toluidine blue staining, and the levels of tumor necrosis factor-a(TNF-α), interleukin-1 β(IL-1β) and interleukin-6(IL-6) in serum and hippocampus were detected by enzyme-linked immunosorbent assay(ELISA). The expression of microglial calcium binding molecule 1(Iba-1) and astroglial fibrillary acidic protein(GFAP) was detected by immunofluorescence, and the expression of Toll-like receptor 4(TLR4)/nuclear factor kB(NF-κB) signaling pathway protein was detected by Western blot(WB). Results Compared with the model group, the high-dose group of calamus tulip decoction could reduce the seizure grade of mice with recurrent epilepsy(P < 0.05), the pyknosis volume of vertebral cells in hippocampal CA3 and CA1 regions of mice in the middle and high dose of CPYJT group and sodium valproate group occasionally decreased, the arrangement was compact and orderly, the expression of Nissl body was increased(P < 0.01), and the levels of TNF-α, IL-1β and IL-6 in serum and hippocampal tissue were significantly decreased(P < 0.01). The number of Iba-1 and GFAP positive cells decreased significantly(P < 0.01), and the expression of TLR4, P-NF-κB and P-IκB-α protein in hippocampal tissue was significantly down-regulated(P < 0.01). Conclusion CPYJT can reduce the susceptibility to epilepsy, improve the pathological changes of epilepsy, inhibit the release of inflammatory factors in serum and hippocampus of mice with recurrent epilepsy, and inhibit the activation of microglia and astrocytes. Its mechanism is related to TLR4/NF-κB signaling pathway. |
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