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| Regulation on the Apoptosis and Related Gene Expression Induced by Myocardial Ischemia-reperfusion |
| Hits 1550 Download times 1637 Received:March 05, 2002 |
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| DOI
10.11656/j.issn.1672-1519.2002.03.24 |
| Key Words
Radix Astragali;Myocardial cell, Ischemia-reperfusion;Apoptosis;bcl-2 and bax |
| Author Name | Affiliation | | Feng Jinping | Tianjin Chest Hospital, Tianjin 300005, China | | Zhao Bingrang | Tianjin Chest Hospital, Tianjin 300005, China | | Lu Yi | 天津医科大学, 天津, 300070 | | 姚智 | 天津医科大学, 天津, 300070 | | 陈书中 | 天津市塘沽医院, 天津, 300450 | | 梁伯平 | Tianjin Chest Hospital, Tianjin 300005, China | | 梁爽霖 | Tianjin Chest Hospital, Tianjin 300005, China |
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| Abstract
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| Objective: To observe the inhibitory effect of Radix Astragali (Huangqi)on apoptosis induced by myocardial ischemia-reperfusion (I/R). Method: By means of ligature and then repatency of left anterior descending branches coronary artery, the rabbit models with myocardial I/R were established. Myocardial DNA electrophoresis, myocardial apoptosis morphology (TUNEL), ultrastructural organization and in situ hybridization of bcl-2 and bax were observed in different situations. Results: The area of myocardial infarction in ischemia group was larger than that in Radix Astragali group and I/R group. The discovery of DNA electrophoresis in I/R group showed a typical apoptosis. The content of DNA in Radix Astragali group was lower than that in I/R group. The TUNEL staining found a few interspersed apoptosis cells in ischemia group, and the sum of apoptosis cells in I/R group was larger than that in Radix Astragali group. The mitochondria and other organellae injury in the I/R group was more serious than that in Radix Astragali group. The expressions of bcl-2 and bax in ischemia group were increased. The expression of bax was increased, but the expression of bcl-2 was decreased in the I/R group. The expression of bax was down regulated and the expression of bcl-2 was up regulated in Radix Astragali group. Conclusion: The myocardial apoptosis can be induced by I/R and ameliovated by Radix Astragali. |
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