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| Influence of Shi’s Rotating Reinforcing and Reducing Method on Correlating Enzymes of Energy Metabolism in Healthy Mice |
| Hits 1924 Download times 2101 Received:June 17, 2002 |
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| DOI
10.11656/j.issn.1672-1519.2002.05.17 |
| Key Words
Shi’s rotating reinforcing and reducing method;energy metabolism;enzyme;mechanism |
| Author Name | Affiliation | | Guan Wei | The First Affiliated Hospital to Tianjin University of TCM, Tianjin 300193, China | | Wang Fang | The First Affiliated Hospital to Tianjin University of TCM, Tianjin 300193, China | | Li Tan | The First Affiliated Hospital to Tianjin University of TCM, Tianjin 300193, China | | 李平 | The First Affiliated Hospital to Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| Objective: To observe the influence of Shi’s rotating reinforcing and reducing method on correlating enzyme activity of energy metabolism in healthy mice. Methods: All Kunming mice were divided into 5 groups. The local tissue of acu-point was skeletal muscle around Zusanli and the distant tissue was stomach. Enzymatic histochemical stain was used to show SDH (succinic dehydrogenase with pearson method), CCO (cytochromeoxidase with seligman method), M-ATP (adenosine triphosphatase with Niles method) and Mg2+-ATP with Wachstein-Meisel method.Results: Compared with control group, Shi’s rotating reinforcing method could elevate the integral luminosity, total area, area percentage of SDH, CCO, ATP markedly (P<0.05), showing that the enzymatic activity was strengthened. While the reducing method could lower them (P<0.05), showing that the enzymatic activity associated with energy metabolism was weakened. Conclusion: The changes of energy metabolism after using Shi’s reinforcing and reducing method may be one of the mechanisms oftemperature effect. |
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