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Determination of Deoxycholic Acid in Porcine Bile Paste by HPLC
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DOI   10.11656/j.issn.1672-1519.2002.06.23
Key Words   porcine deoxycholic acid;porcine bile paste;HPLC
Author NameAffiliation
Xiao Xuefeng Tianjin University of TCM, Tianjin 300193, China 
Chen Xinpei Tianjin University of TCM, Tianjin 300193, China 
Abstract
    Objective: To establish the method for determine the deoxycholic acid in porcine bile paste by HPLC. Method: YWGL C18 chromatographic column (4.6mm×250mm, 10μm) was used. The mobile phase was methanol-water (18:20) with flow rate 1.0 mL per min. Result: The average sample recovery rate was 98.83%. RSD=0.81% (n=6). The linear range was 10-100 μg with α=0.9996. Conclusion: This is a method for quality control of porcine bile paste with simple manipulation and accurate result.

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