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| Osteogenetic process of fibroblast in vitro induced by Shengjiye |
| Hits 1397 Download times 1277 Received:March 04, 2003 |
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| DOI
10.11656/j.issn.1672-1519.2003.02.06 |
| Key Words
fibroblast;osteogenesis;inducing factor |
| Author Name | Affiliation | | LI Xiu-lan | Tianjin Orthopedic Institute, Tianjin 300211, China | | ZHANG Yang | Tianjin Orthopedic Institute, Tianjin 300211, China | | WANG Zeng-li | Tianjin Orthopedic Institute, Tianjin 300211, China | | 师宜健 | Tianjin Orthopedic Institute, Tianjin 300211, China |
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| Abstract
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| [Objective] To explore the inducing factor of osteogenetic process of fibroblast in vitro. [Method] The fibroblasts were isolated and purified from grandlation tissue of New Zealand rabbit and were grown in culture madia containing Shengjiye 60g/L and Fibronectin(FN) 40mg/L.The osteogenetic features of fibrolast were investigated by biochemistry,histochemistry and microscopic observation. [Result] It was found that Shengjiye and FN could stimulate fibroblast to pro-liferate,secrete osteocaline and to synthetize collagen fibrils.The morphological observation found that the fibroblasts transferred from spindle shape to round or multiple,increased in number,confluenced and formed multilayered structure.They were piling up with calcium crystals on their surface and merging gradually into foggy substance.The foggy sub-stances were increasing and forming nodules,which were demonstrated to be new bone by histochemical study with specific labeling of tracycline fluorescence. [Conclusion] Shengjiye can induce the fibroblast in osteogenetic process. |
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