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Content analysis of paeoniarnia glucoside in Jianwei Mixture detected by RP-HPLC |
Hits 1423 Download times 1683 Received:January 03, 2003 |
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DOI
10.11656/j.issn.1672-1519.2003.02.36 |
Key Words
Jianwei Mixture;RP-HPLC;paeoniarnia glucoside |
Author Name | Affiliation | YAN Hong | The First Affiliated Hospital to Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | GUO Wei | The First Affiliated Hospital to Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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Abstract
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[Objective] To detect the content of paeoniarnia glucoside in Jianwei Mixture by RP-HPLC. [Method] Irregular-H C18 chromatographic column was used.The mobile phase was methanol-water(40:60) with detective wave 230nm and flow rate 0.9mL/min. [Result] The separation was well with the linear range in 0.46~2.76μg. [Conclusion] This method is simple,accurate,and reliable and has good producibility for detecting the content of paeoniarnia glucoside in Jianwei Mixture. |
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