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| Influence of Qingnao Yizhi Prescription (QNYZ) on cerebrovascular cells in moyamoya disease |
| Hits 1423 Download times 1842 Received:December 30, 2003 |
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| DOI
10.11656/j.issn.1672-1519.2004.01.09 |
| Key Words
moymoya disease;smooth muscle;endothelial cell;Qingnao Yizhi Prescription (QNYZ) |
| Author Name | Affiliation | | ZHANG Jun-ping | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Bo-li | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | Shanben Qinggao | 日本国东京老人综合研究所神经生物学部 东京 |
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| Abstract
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| [Objective] Moyamoya disease is an infrequent cerebrovascular disease with hereditary tendency and predilection in children. The influence of QNYZ on the proliferation and protein synthesis of cerebrovascular smooth muscle cell of in this disease was observed to study its therapeutic mechanism. [Methods] Human moyamoya smooth muscle cell (HMSMC) strain and human control smooth muscle cell (HCSMC) were used. The cell counter and BrdU incorporation were also chosen. [Result] QNYZ could partially inhibit the proliferation of HCSMC and HMSMC at low concentration (100～200 μg/mL) and show a complete control at high concentration (500～1 000 μg/mL) (P<0.01). It had no marked influence on the proliferation of endothelial cell at each concentration (P>0.05). For DNA synthesis in smooth muscle cell (SMC), BrdU incorporation rate was low (P<0.01) in QNYZ Group. In HCSMC Group, BrdU incorporation rate had no obvious difference between QNYZ Groups with different concentration (P>0.05). In HMSMC Group, QNYZ with the concentration of 1 000 μg/mL could more markedly decrease BrdU incorporation than that of QNYZ at 500 μg/mL with a dose-effect relationship.[Conclusion] QNYZ has no obvious influence on endothelial cell but may inhibit the proliferation of SMC.It can prevent the occurrence of cerebrovascular accident due to moyamoya disease by keeping the completion of tunica intima and maintaining the barrier effect. |
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