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| Bidirectional regulation of Dachengqi Decoction and Rhubarb on immunologic activity of macrophages |
| Hits 1646 Download times 2034 Received:May 10, 2003 |
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| DOI
10.11656/j.issn.1672-1519.2004.01.28 |
| Key Words
Dachengqi Decoction;rhubarb;lipopolysaccharide;macrophages;tumor necrosis factor α |
| Author Name | Affiliation | | YANG Wen-xiu | Department of Biophysics, Nankai University, Tianjin 300071, China | | WANG Hui | Department of Biophysics, Nankai University, Tianjin 300071, China | | LIU Man | Department of Biophysics, Nankai University, Tianjin 300071, China | | 陈立君 | Department of Biophysics, Nankai University, Tianjin 300071, China | | 张莉平 | Department of Biophysics, Nankai University, Tianjin 300071, China | | 刘慧君 | Department of Biophysics, Nankai University, Tianjin 300071, China |
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| Abstract
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| [Objective] To study the effect of Dachengqi Decoction (DCQ) and Rhubarb on the release of tumor necrosis factor α (TNFα) from normal or LPS-stimulated rat peritoneal macrophages (PMΦ). [Methods] TNFαwas assayed by MTT method, using the TNFα-sensitive cell line L929. [Result] The release of TNFαfrom PMΦstimulated by LPS could reach a peak after 8 hours, the peak was increasing with the LPS concentration increasing. Low concentrations of DCQ and Rhubarb could activate normal PMΦto release TNFαslightly. However, DCQ and Rhubarb evidently inhibited the excessive release of TNFαfrom PMΦstimulated by LPS, and the inhibition enhanced with the increasing of drug concentration. The inhibition mostly appeared in early phase after drug administration. [Conclusion] DCQ and Rhubarb have bidirectional regulation on the release of TNFαfrom macrophages. |
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