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| Influence of well acupoints blood-letting therapy on apoptosis associated protein of ischemic brain tissue in rats with acute cerebral ischemia |
| Hits 1784 Download times 1867 Received:February 18, 2004 |
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| DOI
10.11656/j.issn.1672-1519.2004.02.16 |
| Key Words
acute focal cerebral ischemia;well acupoints blood-letting;c-fos protein;HSP70 protein |
| Author Name | Affiliation | | WANG Xiu-yun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | LI Ji-sheng | 武警医学院, 天津, 300162 | | LIU Gong-wang | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 郭义 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 潘荣菁 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 张果忠 | 武警医学院, 天津, 300162 | | 李桂兰 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 韩煜 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 王强 | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the protective function of well acupoints blood-letting method against acute ischemic brain injury.[Methods] Rats were divided into sham operation group,cerebral ischemia group and treatment group. Acute ischemic brain injury was made by middle cerebral artery obstruction(MCAO). Well acupoints blood-letting therapy,one drip each time,was given in rats in treatment group immediately after cerebral ischemia. 1,3,6,24 hours after ischemia,rats were killed and c-fos protein(FOS)and HSP70 immune positive cells of cortex tissue in ischemic region were determined by immunohistochemistry assay to observe the influence on anti-apoptosis gene expression at early stages of acute ischemic brain injury.[Results] Increased expression of c-fos protein and HSP70 protein in cortex tissue of ischemic region was observed with no expression of HSP70 1 hour after ischemia,while the expression of FOS was increased. Expression of FOS and HSP70 tended to increase with the time elapsed during the following 3,6,24 hours. The count of c-fos and HSP70 immune positive cells in treatment group was all higher than that in model group during the same period of time with statistical significance.[Conclusion] Well acupoints blood-letting therapy can obviously enhance the expression of c-fos and HSP70 protein in ischemic region,increase the ability against apoptosis and the tolerance and adaptation ability of nerve cell to hypoxia and ischemia,and enhance the neuron compliance after ischemia,thus further influencing the expression of objective gene at late stages,resisting the development of apoptosis,enhancing the repairing ability of brain. It is suggested that well acupoints blood-letting therapy has certain protective action against early stroke. |
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