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| Influence of extracts from Chinese herbs according to different therapeutic prinples on the differentiation of neural stem cell in vitro |
| Hits 1916 Download times 2856 Received:March 13, 2003 |
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| DOI
10.11656/j.issn.1672-1519.2004.02.34 |
| Key Words
neural stem cell;heat-clearing and fire-purging;supplementing qi and activating blood circulation;differentiation |
| Author Name | Affiliation | | ZHANG Yan-jun | Tianjin University of Traditonal Chinese Medicine, Tianjin 300193, China | | FAN Xiang | Tianjin University of Traditonal Chinese Medicine, Tianjin 300193, China | | HU Li-min | Tianjin University of Traditonal Chinese Medicine, Tianjin 300193, China | | 张师愚 | Tianjin University of Traditonal Chinese Medicine, Tianjin 300193, China | | 张伯礼 | Tianjin University of Traditonal Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To investigate the influence of some extracts from Chinese herbs in different therapeutic principles on the differentiation and maturation of neural stem cell.[Methods] Neural stem cell was isolated from fetal rats rats.The cells were passaged three times.The cultured rat neural stem cells were plated on poly-D-lysin coated 24-well culture plates.β-tubin was used as the marker of immaturity neuron,tau as the marker of maturity neuron. The expression of β-tubin and tau mRNA were analyzed with RT-PCR method.The influence of baicalin,jasminoidin,Astragaloside IV Notoginsenoside extracted from Chinese herbs in different therapeutic principles on the differentiation and maturation was investigated.[Results] Baicilin and jasminoidin could induce neural stem cells differentiated to neuron specificity,while combined with Astragaloside IV and Notoginsenoside could promote the maturation of the differentiated neuron.[Conclusion] The comined use of Chinese herbs in differentiated therapeutic principles can promote the neuranagenesis. |
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