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| Effect of Zhuanggu Zhitong Capsule on the proliferation and differentiation in rat of osteoblast |
| Hits 1796 Download times 1658 Received:March 25, 2004 |
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| DOI
10.11656/j.issn.1672-1519.2004.03.34 |
| Key Words
osteoblast;Zhuanggu Zhitong capsule(ZZC);Serum with absorbed Zhuanggu Zhitong capsule;proliferation;differentiation. |
| Author Name | Affiliation | | LEI Xiao-ming | Hunan University of TCM, Changsha, 410007, China | | TIAN Song | Hunan University of TCM, Changsha, 410007, China |
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| Abstract
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| [Objective]To observe the effect of Zhuanggu Zhitong capsule(ZZC)on the proliferation and differentiation in rat of osteoblast,and provide experimental evidence on the mechanism for treatment of osteoporosis.[Methods]The second generation of rat osteoblast was cultured in medium with 40% ZZC-contained serum,and cell growth and the formation of calcium nodule were observed.Growth curve of osteoblast was detected by MTT method,while differentiation and prolif-eration were determined by measuring alkalinephosphtase(ALP)activity.[Result]Serum with absorbed ZZC obviously in-creased osteoblast proliferation and differentiation and the secretion of ALP.[Conclusion]Serum with absorbed ZZC can promote the proliferation and differentiation of osteoblast in rats.It is probably the mechanism in preventing and curing the osteoporosis. |
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